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SOX4 Recombinant Matched Antibody Pair, PBS Only

Host / Isotype

Capture: Rabbit / IgG

Detection: Rabbit / IgG

Reactivity

Capture: human

Detection: human

Applications

Cytometric bead array, Sandwich ELISA

Range

Cytometric Bead Array: 0.625-80 ng/mL

Sandwich ELISA: 31.3-2000 pg/mL

Cat No : MP00619-1

Print datasheet

Synonyms

EVI16, SOX4, Transcription factor SOX 4

Validation Data Gallery

  • Sandwich ELISA standard curve of MP00619-1, Human SOX4 Recombinant Matched Antibody Pair - PBS only. 83660-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag26583. 83660-3-PBS was HRP conjugated as the detection antibody. Range: 31.3-2000 pg/mL.

    Sandwich ELISA standard curve of MP00619-1

    Sandwich ELISA standard curve of MP00619-1, Human SOX4 Recombinant Matched Antibody Pair - PBS only. 83660-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag26583. 83660-3-PBS was HRP conjugated as the detection antibody. Range: 31.3-2000 pg/mL.

  • The mean SOX4 concentration was determined to be 16.1 ng/mL in HepG2 cell extract based on a 1.2 mg/mL extract load.

    Sample test of MP00619-1

    The mean SOX4 concentration was determined to be 16.1 ng/mL in HepG2 cell extract based on a 1.2 mg/mL extract load.

  • Cytometric bead array standard curve of MP00619-1, SOX4 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83660-1-PBS. Detection antibody: 83660-3-PBS. Standard: Ag26583. Range: 0.625-80 ng/mL.

    Cytometric bead array standard curve of MP00619-1

    Cytometric bead array standard curve of MP00619-1, SOX4 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83660-1-PBS. Detection antibody: 83660-3-PBS. Standard: Ag26583. Range: 0.625-80 ng/mL.

Tested Applications

Cytometric bead array, Sandwich ELISA

Recommended dilution

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

产品信息

MP00619-1 targets SOX4 in immunoassays as a matched antibody pair. Validated in Cytometric bead array, Sandwich ELISA.

Range: 0.625-80 ng/mL (Cytometric Bead Array); 31.3-2000 pg/mL (Sandwich ELISA);

Capture antibody: SOX4 Recombinant antibody, PBS Only (Capture) 83660-1-PBS (240625E3). 100 μg. Concentration 1 mgl/ml.

Detection antibody: SOX4 Recombinant antibody, PBS Only (Detector) 83660-3-PBS (240625F10). 100 μg. Concentration 1 mgl/ml.

Alternative SOX4 matched antibody pairs: MP00619-2

Unconjugated rabbit recombinant monoclonal antibody pair in PBS only storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

Matched antibody pairs are designed for use in a variety of assays and platforms that require matched antibody pairs. These include:
Sandwich ELISA
Multiplex bead-based arrays
FRET/TR-FRET/HTRF
Simoa® immunoassays (registered trademark of Quanterix, Inc)
AlphaLISA® (registered trademark of PerkinElmer, Inc)
Meso Scale Discovery® (registered trademark of Meso Scale Diagnostics, LLC)
ELLA™ Automated Immunoassay (registered trademark of Bio-Techne Corporation)

Please note we have not tested this product in the above platforms, they are listed for suggestion only as applications for matched antibody pairs. Antibody use should be optimized for each application and assay.

Catalog Number 83660-1-PBS
Tested Reactivity human
Immunogen SOX4 fusion protein Ag26583
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name SRY (sex determining region Y)-box 4
Synonyms Transcription factor SOX-4, EVI16, CSS10, 240625E3
GenBank accession numberBC072668
Gene symbol SOX4
UniprotQ06945
Gene ID (NCBI) 6659
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C
Catalog Number 83660-3-PBS
Tested Reactivity human
Immunogen SOX4 fusion protein Ag26583
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name SRY (sex determining region Y)-box 4
Synonyms Transcription factor SOX-4, EVI16, CSS10, 240625F10
GenBank accession numberBC072668
Gene symbol SOX4
UniprotQ06945
Gene ID (NCBI) 6659
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C