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SOX4 Recombinant antibody, PBS Only (Capture)

SOX4 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

240625E3

Cat no : 83660-1-PBS

Print datasheet

Synonyms

Transcription factor SOX-4, EVI16, CSS10, 240625E3

验证数据展示

  • FC experiment of SH-SY5Y using 83660-1-RR (same clone as 83660-1-PBS)

    FC experiment of SH-SY5Y using 83660-1-RR (same clone as 83660-1-PBS)

    1x10^6 SH-SY5Y cells were intracellularly stained with 0.25 ug SOX4 Recombinant antibody (83660-1-RR, Clone:240625E3) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 83660-1-PBS in a different storage buffer formulation.

  • WB analysis using 83660-1-RR (same clone as 83660-1-PBS)

    WB analysis using 83660-1-RR (same clone as 83660-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83660-1-RR (SOX4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83660-1-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP00619-1

    Sandwich ELISA standard curve of MP00619-1

    Sandwich ELISA standard curve of MP00619-1, Human SOX4 Recombinant Matched Antibody Pair - PBS only. 83660-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag26583. 83660-3-PBS was HRP conjugated as the detection antibody. Range: 31.3-2000 pg/mL.

  • Sample test of MP00619-1

    Sample test of MP00619-1

    The mean SOX4 concentration was determined to be 16.1 ng/mL in HepG2 cell extract based on a 1.2 mg/mL extract load.

  • Cytometric bead array standard curve of MP00619-1

    Cytometric bead array standard curve of MP00619-1

    Cytometric bead array standard curve of MP00619-1, SOX4 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83660-1-PBS. Detection antibody: 83660-3-PBS. Standard: Ag26583. Range: 0.625-80 ng/mL.

  • Cytometric bead array standard curve of MP00619-2

    Cytometric bead array standard curve of MP00619-2

    Cytometric bead array standard curve of MP00619-2, SOX4 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83660-1-PBS. Detection antibody: 83660-2-PBS. Standard: Ag26583. Range: 0.625-80 ng/mL.

  • Affinity and Kinetic Characterization of 83660-1-RR

    Affinity and Kinetic Characterization of 83660-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83660-1-RR against Human SOX4 were performed. The affinity constant is below 1 pM.

产品信息

83660-1-PBS targets SOX4 as part of a matched antibody pair:

MP00619-1: 83660-1-PBS capture and 83660-3-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

MP00619-2: 83660-1-PBS capture and 83660-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications WB, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test Application Description
Tested Reactivity human
Immunogen SOX4 fusion protein Ag26583 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name SRY (sex determining region Y)-box 4
Synonyms Transcription factor SOX-4, EVI16, CSS10, 240625E3
Calculated Molecular Weight 474 aa, 47 kDa
GenBank Accession NumberBC072668
Gene Symbol SOX4
Gene ID (NCBI) 6659
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ06945
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C