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IL1RAP Recombinant Matched Antibody Pair, PBS Only

Host / Isotype

Capture: Rabbit / IgG

Detection: Rabbit / IgG

Reactivity

Capture: human

Detection: human

Applications

Sandwich ELISA

Range

Sandwich ELISA: 7.8-500 pg/mL

Cat No : MP00708-4

Print datasheet

Synonyms

Validation Data Gallery

  • Sandwich ELISA standard curve of MP00708-4, Human IL1RAP Recombinant Matched Antibody Pair - PBS only. 98073-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0827. 83726-5-PBS was HRP conjugated as the detection antibody. Range: 7.8-500 pg/mL

    Sandwich ELISA standard curve of MP00708-4

    Sandwich ELISA standard curve of MP00708-4, Human IL1RAP Recombinant Matched Antibody Pair - PBS only. 98073-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0827. 83726-5-PBS was HRP conjugated as the detection antibody. Range: 7.8-500 pg/mL

  • Serum of six individual healthy human donors was measured. The human IL1RAP concentration of detected samples was determined to be 21.67 pg/mL with a range of 7.89 - 42.37 pg/mL.

    Sample test of MP00708-4

    Serum of six individual healthy human donors was measured. The human IL1RAP concentration of detected samples was determined to be 21.67 pg/mL with a range of 7.89 - 42.37 pg/mL.

  • Plasma of six individual healthy human donors was measured. The human IL1RAP concentration of detected samples was determined to be 266.79 pg/mL with a range of 13.79 - 1,302.80 pg/mL.

    Sample test of MP00708-4

    Plasma of six individual healthy human donors was measured. The human IL1RAP concentration of detected samples was determined to be 266.79 pg/mL with a range of 13.79 - 1,302.80 pg/mL.

Tested Applications

Sandwich ELISA

Recommended dilution

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

产品信息

MP00708-4 targets IL1RAP in immunoassays as a matched antibody pair. Validated in Sandwich ELISA.

Range: 7.8-500 pg/mL (Sandwich ELISA);

Capture antibody: Anti-Human IL1RAP Rabbit Recombinant Antibody, PBS Only 98073-1-PBS (240756A12). 100 μg. Concentration 1 mgl/ml.

Detection antibody: IL1RAP Recombinant antibody, PBS Only (Detector) 83726-5-PBS (240756E7). 100 μg. Concentration 1 mgl/ml.

Alternative IL1RAP matched antibody pairs: MP00708-1, MP00708-2, MP00708-3

Unconjugated rabbit recombinant monoclonal antibody pair in PBS only storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

Matched antibody pairs are designed for use in a variety of assays and platforms that require matched antibody pairs. These include:
Sandwich ELISA
Multiplex bead-based arrays
FRET/TR-FRET/HTRF
Simoa® immunoassays (registered trademark of Quanterix, Inc)
AlphaLISA® (registered trademark of PerkinElmer, Inc)
Meso Scale Discovery® (registered trademark of Meso Scale Diagnostics, LLC)
ELLA™ Automated Immunoassay (registered trademark of Bio-Techne Corporation)

Please note we have not tested this product in the above platforms, they are listed for suggestion only as applications for matched antibody pairs. Antibody use should be optimized for each application and assay.

Catalog Number 98073-1-PBS
Tested Reactivity human
Immunogen Fusion Protein
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name interleukin 1 receptor accessory protein
Synonyms IL 1RAcP, IL 1R3, IL 1R 3, EC:3.2.2.6, C3orf13
GenBank accession numberNM_002182.4
Gene symbol IL1RAP
UniprotQ9NPH3-1
Gene ID (NCBI) 3556
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purfication
Storage Buffer PBS only
Storage Conditions Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C
Catalog Number 83726-5-PBS
Tested Reactivity human
Immunogen Fusion Protein
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name interleukin 1 receptor accessory protein
Synonyms IL 1RAcP, IL 1R3, IL 1R 3, EC:3.2.2.6, C3orf13
GenBank accession numberNM_002182.4
Gene symbol IL1RAP
UniprotQ9NPH3-1
Gene ID (NCBI) 3556
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C