• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

PARK7,DJ-1 Hybrid Matched Antibody Pair, PBS Only

Host / Isotype

Capture: Rabbit / IgG

Detection: Mouse / IgG2a

Reactivity

Capture: human

Detection: human

Applications

Sandwich ELISA

Range

Sandwich ELISA: 0.39-25 ng/mL

Cat No : MP80004-1

Print datasheet

Synonyms

DJ 1, DJ1, FLJ27376, FLJ34360, FLJ92274, Oncogene DJ1, PARK7, Parkinson disease protein 7, Protein DJ 1

Validation Data Gallery

  • Sandwich ELISA standard curve of MP80004-1, Human PARK7/DJ-1 Monoclonal Matched Antibody Pair - PBS only. 82913-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag2287. 68802-1-PBS was HRP conjugated as the detection antibody. Range: 0.39-25 ng/mL.

    Sandwich ELISA standard curve of MP80004-1

    Sandwich ELISA standard curve of MP80004-1, Human PARK7/DJ-1 Monoclonal Matched Antibody Pair - PBS only. 82913-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag2287. 68802-1-PBS was HRP conjugated as the detection antibody. Range: 0.39-25 ng/mL.

  • Serum of sixteen individual healthy human donors were measured. The human PARK7/DJ-1 concentration of detected samples was determined to be 28.3 ng/mL with a range of 6.3 - 44.6 ng/mL.

    Sample test of MP80004-1

    Serum of sixteen individual healthy human donors were measured. The human PARK7/DJ-1 concentration of detected samples was determined to be 28.3 ng/mL with a range of 6.3 - 44.6 ng/mL.

  • The mean human PARK7/DJ-1 concentration was determined to be 14.25 ng/mL in HL-60 cell extract based on a 4.3 mg/mL extract load.

    Sample test of MP80004-1

    The mean human PARK7/DJ-1 concentration was determined to be 14.25 ng/mL in HL-60 cell extract based on a 4.3 mg/mL extract load.

  • The human PARK7/DJ-1 concentration of Human cerebrospinal fluid (CSF) samples were determined to be 8.3 ng/mL.

    Sample test of MP80004-1

    The human PARK7/DJ-1 concentration of Human cerebrospinal fluid (CSF) samples were determined to be 8.3 ng/mL.

Tested Applications

Sandwich ELISA

Recommended dilution

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

产品信息

MP80004-1 targets PARK7,DJ-1 in immunoassays as a matched antibody pair. Validated in Sandwich ELISA.

Range: 0.39-25 ng/mL (Sandwich ELISA);

Capture antibody: PARK7/DJ-1 Recombinant antibody, PBS Only (Capture) 82913-1-PBS (230124E12). 100 μg. Concentration 1 mgl/ml.

Detection antibody: PARK7/DJ-1 Monoclonal antibody, PBS Only (Detector) 68802-1-PBS (1E5C3). 100 μg. Concentration 1 mgl/ml.

Unconjugated rabbit recombinant antibody and mouse monoclonal antibody pair in PBS only storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Mouse monoclonal antibody and Rabbit Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

Matched antibody pairs are designed for use in a variety of assays and platforms that require matched antibody pairs. These include:
Sandwich ELISA
Multiplex bead-based arrays
FRET/TR-FRET/HTRF
Simoa® immunoassays (registered trademark of Quanterix, Inc)
AlphaLISA® (registered trademark of PerkinElmer, Inc)
Meso Scale Discovery® (registered trademark of Meso Scale Diagnostics, LLC)
ELLA™ Automated Immunoassay (registered trademark of Bio-Techne Corporation)

Please note we have not tested this product in the above platforms, they are listed for suggestion only as applications for matched antibody pairs. Antibody use should be optimized for each application and assay.

Catalog Number 82913-1-PBS
Tested Reactivity human
Immunogen PARK7/DJ-1 fusion protein Ag2287
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name Parkinson disease (autosomal recessive, early onset) 7
Synonyms PARK7, PARK7,DJ-1, Maillard deglycase, EC:3.1.2.-, EC:3.5.1.-, EC:3.5.1.124, DJ-1
GenBank accession numberBC008188
Gene symbol PARK7
UniprotQ99497
Gene ID (NCBI) 11315
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C
Catalog Number 68802-1-PBS
Tested Reactivity human
Immunogen PARK7/DJ-1 fusion protein Ag2287
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name Parkinson disease (autosomal recessive, early onset) 7
Synonyms PARK7, PARK7,DJ-1, Maillard deglycase, EC:3.1.2.-, EC:3.5.1.-, EC:3.5.1.124, DJ-1
GenBank accession numberBC008188
Gene symbol PARK7
UniprotQ99497
Gene ID (NCBI) 11315
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C