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β-Nicotinamide Mononucleotide Monoclonal antibody

β-Nicotinamide Mononucleotide Monoclonal Antibody for ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

ELISA

Conjugate

Unconjugated

CloneNo.

3A1F11

Cat No : 68638-1-Ig

Print datasheet

Synonyms

NMN, β NMN, β-Nicotinamide Mononucleotide, β-NMN

验证数据展示

  • ELISA experiment of β-Nicotinamide Mononucleotide using 68638-1-Ig

    ELISA experiment of β-Nicotinamide Mononucleotide using 68638-1-Ig

    Indirect ELISA and competitive ELISA results show that this antibody is specific to β-Nicotinamide Mononucleotide (NMN). Indirect ELISA was performed by coating BSA conjugated β-Nicotinamide Mononucleotide (NMN) at ~10ng/well followed by blocking with 1% BSA. Serial diluted primary antibody was added to the plates and incubated at 37℃. HRP-goat anti-mouse was used for detection. Competitive ELISA was performed similarly except that different concentration of β-Nicotinamide Mononucleotide (NMN) were mixed in primary antibody (fixed at 10 ng/mL).

  • ELISA experiment of urine using 68638-1-Ig

    ELISA experiment of urine using 68638-1-Ig

    BSA conjugated β-Nicotinamide Mononucleotide (NMN) was coated in 96 well plate at ~10 ng/well (by NMN amount), followed by blocking with 1% BSA. Different concentrations of NMN standard as well as diluted urine from NMN orally administrated mouse were mixed with 10 ng/mL β-Nicotinamide Mononucleotide antibody 68638-1-Ig respectively. Urine NMN was calculated based on standard curve.

经过测试的应用

Positive ELISA detected inβ-Nicotinamide Mononucleotide, urine

推荐稀释比

ApplicationDilution
Enzyme-linked Immunosorbent Assay (ELISA)ELISA : 1:2000-1:20000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68638-1-Ig targets β-Nicotinamide Mononucleotide in ELISA applications and shows reactivity with Human samples.

Tested Applications ELISA Application Description
Tested Reactivity Human
Immunogen PTG 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name β-Nicotinamide Mononucleotide
Synonyms NMN, β NMN, β-Nicotinamide Mononucleotide, β-NMN
Gene Symbol
Gene ID (NCBI)
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Nicotinamide mononucleotide ("NMN" and "β-NMN") is a nucleotide derived from ribose, nicotinamide, nicotinamide riboside and niacin. In humans, several enzymes use NMN to generate nicotinamide adenine dinucleotide (NADH). In mice, it has been proposed that NMN is absorbed via the small intestine within 10 minutes of oral uptake and converted to nicotinamide adenine dinucleotide (NAD+) through the Slc12a8 transporter. However, this observation has been challenged, and the matter remains unsettled. Maintain cell quality and reduce cell aging with β-nicotinamide mononucleotide (β-NMN), an intermediate in the biosynthesis of nicotinamide adenine dinucleotide (NAD+) (Bogan & Brenner). β-NMN is a product of the nicotinamide phosphoribosyltransferase (NAMPT) reaction and is converted to NAD+ by nicotinamide-nucleotide adenylyltransferase.