Indirect ELISA and competitive ELISA results show that this antibody is specific to β-Nicotinamide Mononucleotide (NMN). Indirect ELISA was performed by coating BSA conjugated β-Nicotinamide Mononucleotide (NMN) at ~10ng/well followed by blocking with 1% BSA. Serial diluted primary antibody was added to the plates and incubated at 37℃. HRP-goat anti-mouse was used for detection. Competitive ELISA was performed similarly except that different concentration of β-Nicotinamide Mononucleotide (NMN) were mixed in primary antibody (fixed at 10 ng/mL). This data was developed using the same antibody clone with 68638-1-PBS in a different storage buffer formulation.

BSA conjugated β-Nicotinamide Mononucleotide (NMN) was coated in 96 well plate at ~10 ng/well (by NMN amount), followed by blocking with 1% BSA. Different concentrations of NMN standard as well as diluted urine from NMN orally administrated mouse were mixed with 10 ng/mL β-Nicotinamide Mononucleotide antibody 68638-1-Ig respectively. Urine NMN was calculated based on standard curve. This data was developed using the same antibody clone with 68638-1-PBS in a different storage buffer formulation.