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14-3-3 Monoclonal antibody, PBS Only

14-3-3 Monoclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, rat, mouse, pig

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2E10A3

Cat no : 66061-1-PBS

Print datasheet

Synonyms

14 3 3, 14 3 3 protein T cell, 14 3 3 protein tau, 14 3 3 protein theta, 14-3-3, 14-3-3 tau, HS1, Protein HS1, YWHAQ

验证数据展示

  • WB analysis using 66061-1-Ig (same clone as 66061-1-PBS)

    WB analysis using 66061-1-Ig (same clone as 66061-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66061-1-Ig (14-3-3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66061-1-PBS in a different storage buffer formulation.

  • IP experiment of rat brain using 66061-1-Ig (same clone as 66061-1-PBS)

    IP experiment of rat brain using 66061-1-Ig (same clone as 66061-1-PBS)

    IP result of anti-14-3-3 (IP:66061-1-Ig, 4ug; Detection:66061-1-Ig 1:20000) with rat brain tissue lysate 1080 ug. This data was developed using the same antibody clone with 66061-1-PBS in a different storage buffer formulation.

  • IHC staining of human lung cancer using 66061-1-Ig (same clone as 66061-1-PBS)

    IHC staining of human lung cancer using 66061-1-Ig (same clone as 66061-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66061-1-Ig (14-3-3 Antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66061-1-PBS in a different storage buffer formulation.

  • IHC staining of human lung cancer using 66061-1-Ig (same clone as 66061-1-PBS)

    IHC staining of human lung cancer using 66061-1-Ig (same clone as 66061-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66061-1-Ig (14-3-3 Antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66061-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 66061-1-Ig (same clone as 66061-1-PBS)

    IF Staining of HepG2 using 66061-1-Ig (same clone as 66061-1-PBS)

    Immunofluorescent analysis of HepG2 cells using 66061-1-Ig (14-3-3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG (H+L). This data was developed using the same antibody clone with 66061-1-PBS in a different storage buffer formulation.

产品信息

66061-1-PBS targets 14-3-3 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, rat, mouse, pig samples.

Tested Applications WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
Tested Reactivity human, rat, mouse, pig
Immunogen 14-3-3 fusion protein Ag6055 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, theta polypeptide
Synonyms 14 3 3, 14 3 3 protein T cell, 14 3 3 protein tau, 14 3 3 protein theta, 14-3-3, 14-3-3 tau, HS1, Protein HS1, YWHAQ
Calculated Molecular Weight 28 kDa
Observed Molecular Weight 31 kDa, 28 kDa
GenBank Accession NumberBC056867
Gene Symbol 14-3-3 theta
Gene ID (NCBI) 10971
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP27348
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

14-3-3 proteins interact with a wide spectrum of proteins and possess diverse functions. Mammals express seven distinct 14-3-3 isoforms (gamma, epsilon, beta, zeta, sigma, theta, tau) that form multiple homo- and hetero- dimmers. 14-3-3 proteins display the highest expression levels in the brain, and have been implicated in several neurodegenerative diseases, including Alzheimer's disease and amyotrophic lateral sclerosis.