验证数据展示
经过测试的应用
Positive WB detected in | HEK-293 cells, HeLa cells, HepG2 cells, human brain tissue, pig brain tissue, rat brain tissue, mouse brain tissue |
Positive IP detected in | rat brain tissue |
Positive IHC detected in | human lung cancer tissue, rat brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
应用 | 推荐稀释比 |
---|---|
Western Blot (WB) | WB : 1:2000-1:20000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
66061-1-Ig targets 14-3-3 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, rat, mouse, pig samples.
经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
文献引用应用 | WB, IP, IF, IHC, CoIP |
经测试反应性 | human, rat, mouse, pig |
文献引用反应性 | human, mouse, rat |
免疫原 | 14-3-3 fusion protein Ag6055 种属同源性预测 |
宿主/亚型 | Mouse / IgG1 |
抗体类别 | Monoclonal |
产品类型 | Antibody |
全称 | tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, theta polypeptide |
别名 | 14 3 3, 14 3 3 protein T cell, 14 3 3 protein tau, 14 3 3 protein theta, 14-3-3, 14-3-3 tau, HS1, Protein HS1, YWHAQ |
计算分子量 | 28 kDa |
观测分子量 | 31 kDa, 28 kDa |
GenBank蛋白编号 | BC056867 |
基因名称 | 14-3-3 theta |
Gene ID (NCBI) | 10971 |
RRID | AB_11042772 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Protein G purification |
UNIPROT ID | P27348 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
14-3-3 proteins interact with a wide spectrum of proteins and possess diverse functions. Mammals express seven distinct 14-3-3 isoforms (gamma, epsilon, beta, zeta, sigma, theta, tau) that form multiple homo- and hetero- dimmers. 14-3-3 proteins display the highest expression levels in the brain, and have been implicated in several neurodegenerative diseases, including Alzheimer's disease and amyotrophic lateral sclerosis.
实验方案
Product Specific Protocols | |
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WB protocol for 14-3-3 antibody 66061-1-Ig | Download protocol |
IHC protocol for 14-3-3 antibody 66061-1-Ig | Download protocol |
IF protocol for 14-3-3 antibody 66061-1-Ig | Download protocol |
IP protocol for 14-3-3 antibody 66061-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Aging (Albany NY) In vitro and in vivo efficacy of the novel oral proteasome inhibitor NNU546 in multiple myeloma. | ||
Neurosci Res HAP1 interacts with 14-3-3 to regulate epileptic seizure via GABAAR-mediated inhibitory synaptic transmission in pentylenetetrazole rat model. | ||
Clin Nutr Ceramide enhanced the hepatic glucagon response through regulation of CREB activity |