14-3-3 Monoclonal antibody

14-3-3 Monoclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 66061-1-Ig

产品说明书

CloneNo. 2E10A3

宿主/亚型

Mouse / IgG1

种属反应性

human, rat, mouse, pig

应用

WB, IHC, IF/ICC, IP, ELISA and More (2)

14 3 3, 14 3 3 protein T cell, 14 3 3 protein tau, 14 3 3 protein theta, 14-3-3, 14-3-3 tau, HS1, Protein HS1, YWHAQ

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, HepG2 cells, human brain tissue, pig brain tissue, rat brain tissue, mouse brain tissue
Positive IP detected inrat brain tissue
Positive IHC detected inhuman lung cancer tissue, rat brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:20000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66061-1-Ig targets 14-3-3 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, rat, mouse, pig samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
文献引用应用WB, IP, IF, IHC, CoIP
经测试反应性 human, rat, mouse, pig
文献引用反应性human, mouse, rat
免疫原 14-3-3 fusion protein Ag6055 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, theta polypeptide
别名 14 3 3, 14 3 3 protein T cell, 14 3 3 protein tau, 14 3 3 protein theta, 14-3-3, 14-3-3 tau, HS1, Protein HS1, YWHAQ
计算分子量 28 kDa
观测分子量 31 kDa, 28 kDa
GenBank蛋白编号BC056867
基因名称 14-3-3 theta
Gene ID (NCBI) 10971
RRIDAB_11042772
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP27348
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

14-3-3 proteins interact with a wide spectrum of proteins and possess diverse functions. Mammals express seven distinct 14-3-3 isoforms (gamma, epsilon, beta, zeta, sigma, theta, tau) that form multiple homo- and hetero- dimmers. 14-3-3 proteins display the highest expression levels in the brain, and have been implicated in several neurodegenerative diseases, including Alzheimer's disease and amyotrophic lateral sclerosis.

实验方案

Product Specific Protocols
WB protocol for 14-3-3 antibody 66061-1-IgDownload protocol
IHC protocol for 14-3-3 antibody 66061-1-IgDownload protocol
IF protocol for 14-3-3 antibody 66061-1-IgDownload protocol
IP protocol for 14-3-3 antibody 66061-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIHC

Aging (Albany NY)

In vitro and in vivo efficacy of the novel oral proteasome inhibitor NNU546 in multiple myeloma.

Authors - Hui Zhou
humanIF

Front Immunol

Interactome of the Autoimmune Risk Protein ANKRD55.

Authors - Nerea Ugidos
human,ratWB,CoIP

Neurosci Res

HAP1 interacts with 14-3-3 to regulate epileptic seizure via GABAAR-mediated inhibitory synaptic transmission in pentylenetetrazole rat model.

Authors - Yuetao Wen
mouseWB,IP

Clin Nutr

Ceramide enhanced the hepatic glucagon response through regulation of CREB activity

Authors - Jizheng Wang
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