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验证数据展示

  • IF Staining of HepG2 using CL594-66061

    IF Staining of HepG2 using CL594-66061

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CL594-66061 (14-3-3 antibody) at dilution of 1:50.

CoraLite®594-conjugated 14-3-3 Monoclonal antibody

14-3-3 Monoclonal Antibody for IF/ICC
Cat No. CL594-66061

产品说明书

CloneNo. 2E10A3

宿主/亚型

Mouse / IgG1

种属反应性

human, rat, mouse, pig

应用

IF/ICC

别名

14 3 3, 14 3 3 protein T cell, 14 3 3 protein tau, 14 3 3 protein theta, 1C5, HS1, Protein HS1, YWHAQ

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite®594
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 66061-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-66061 targets 14-3-3 in IF/ICC applications and shows reactivity with human, rat, mouse, pig samples.

经测试应用 IF/ICC Application Description
经测试反应性 human, rat, mouse, pig
免疫原 14-3-3 fusion protein Ag6055 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, theta polypeptide
别名 14 3 3, 14 3 3 protein T cell, 14 3 3 protein tau, 14 3 3 protein theta, 1C5, HS1, Protein HS1, YWHAQ
计算分子量 28 kDa
观测分子量 31 kDa, 28 kDa
GenBank蛋白编号BC056867
基因名称 14-3-3 theta
Gene ID (NCBI) 10971
RRIDAB_2883498
偶联类型 CoraLite®594 Fluorescent Dye
最大激发/发射波长588 nm / 604 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP27348
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

14-3-3 proteins interact with a wide spectrum of proteins and possess diverse functions. Mammals express seven distinct 14-3-3 isoforms (gamma, epsilon, beta, zeta, sigma, theta, tau) that form multiple homo- and hetero- dimmers. 14-3-3 proteins display the highest expression levels in the brain, and have been implicated in several neurodegenerative diseases, including Alzheimer's disease and amyotrophic lateral sclerosis.

实验方案

Product Specific Protocols
IF protocol for CL594 14-3-3 antibody CL594-66061Download protocol
Standard Protocols
Click here to view our Standard Protocols