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验证数据展示

  • IF Staining of HepG2 using CL594-66061

    IF Staining of HepG2 using CL594-66061

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CL594-66061 (14-3-3 antibody) at dilution of 1:50.

CoraLite®594-conjugated 14-3-3 Monoclonal antibody

14-3-3 Monoclonal Antibody for IF/ICC
Cat No. CL594-66061

Print datasheet

CloneNo. 2E10A3

Host / Isotype

Mouse / IgG1

Reactivity

human, rat, mouse, pig

Applications

IF/ICC

Synonyms

14 3 3, 14 3 3 protein T cell, 14 3 3 protein tau, 14 3 3 protein theta, 1C5, HS1, Protein HS1, YWHAQ

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite®594
Size: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 66061-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-66061 targets 14-3-3 in IF/ICC applications and shows reactivity with human, rat, mouse, pig samples.

Tested Applications IF/ICC Application Description
Tested Reactivity human, rat, mouse, pig
Immunogen 14-3-3 fusion protein Ag6055 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, theta polypeptide
Synonyms 14 3 3, 14 3 3 protein T cell, 14 3 3 protein tau, 14 3 3 protein theta, 1C5, HS1, Protein HS1, YWHAQ
Calculated Molecular Weight 28 kDa
Observed Molecular Weight 31 kDa, 28 kDa
GenBank Accession NumberBC056867
Gene Symbol 14-3-3 theta
Gene ID (NCBI) 10971
RRIDAB_2883498
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP27348
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

14-3-3 proteins interact with a wide spectrum of proteins and possess diverse functions. Mammals express seven distinct 14-3-3 isoforms (gamma, epsilon, beta, zeta, sigma, theta, tau) that form multiple homo- and hetero- dimmers. 14-3-3 proteins display the highest expression levels in the brain, and have been implicated in several neurodegenerative diseases, including Alzheimer's disease and amyotrophic lateral sclerosis.

实验方案

Product Specific Protocols
IF protocol for CL594 14-3-3 antibody CL594-66061Download protocol
Standard Protocols
Click here to view our Standard Protocols