• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • FC experiment of NIH/3T3 using CL555-14503

    FC experiment of NIH/3T3 using CL555-14503

    1X10^6 NIH/3T3 cells were intracellularly stained with 0.2 ug CoraLite® Plus 555 Anti-Human 14-3-3 (CL555-14503) (red), or 0.2 ug CoraLite® Plus 555 Rabbit IgG Control Antibody(CL555-30000)(Blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Featured Product
  • KD/KO验证

CoraLite® Plus 555-conjugated 14-3-3 Polyclonal antibody

14-3-3 Polyclonal Antibody for FC (Intra)
Cat No. CL555-14503

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

FC (Intra)

别名

14 3 3, 14 3 3 protein T cell, 14 3 3 protein tau, 14 3 3 protein theta, 14-3-3, 1C5, HS1, Protein HS1, YWHAQ

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 555
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive FC (Intra) detected inNIH/3T3 cells
Positive FC detected inNIH/3T3 cells
For other applications, we recommend the unconjugated version of this antibody, 14503-1-AP

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL555-14503 targets 14-3-3 in FC (Intra) applications and shows reactivity with human, mouse, rat samples.

经测试应用 FC (Intra) Application Description
经测试反应性 human, mouse, rat
免疫原 14-3-3 fusion protein Ag5959 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, theta polypeptide
别名 14 3 3, 14 3 3 protein T cell, 14 3 3 protein tau, 14 3 3 protein theta, 14-3-3, 1C5, HS1, Protein HS1, YWHAQ
计算分子量 28 kDa
观测分子量 31 kDa
GenBank蛋白编号BC056867
基因名称 14-3-3 theta
Gene ID (NCBI) 10971
RRIDAB_2934637
偶联类型 CoraLite® Plus 555 Fluorescent Dye
最大激发/发射波长554 nm / 570 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP27348
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

14-3-3 proteins are the first phosphoserine/phosphothreonine-binding proteins to be discovered. 14-3-3 family members interact with a wide spectrum of proteins and possess diverse functions. Mammals express seven distinct 14-3-3 isoforms (gamma, epsilon, beta, zeta, sigma, theta, tau) that form multiple homo- and hetero- dimmers. 14-3-3 proteins display the highest expression levels in the brain, and have been implicated in several neurodegenerative diseases, including Alzheimer's disease and amyotrophic lateral sclerosis.

实验方案

Product Specific Protocols
FC protocol for CL Plus 555 14-3-3 antibody CL555-14503Download protocol
Standard Protocols
Click here to view our Standard Protocols