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5-Formylcytosine (5-fC) antibody (pAb)

Host / Isotype

Rabbit / Serum

Reactivity

Not Species Specific

Applications

DB, ICC, IF, IHC, WB

Cat No : 61223,61224 61223

Synonyms

5-Formylcytidine, 5-formylcytosine, 5-fC, 5fc, TET, dna methylation, stem cell, stem cells, embryogenesis, sample

验证数据展示

  • Dot blot analysis was used to confirm the specificity of 5-Formylcytosine antibody for 5-Formylcytidine Various BSA conjugated nucleosides were spotted onto PVDF membrane and probed with the antibody at 1:10,000. Column 1: 5-fC. Column. 2: 5-caC. Column. 3: 5-hmC. Column. 4: Cytidine. Column. 5: 5-mC.

    Dot blot analysis was used to confirm the specificity of 5-Formylcytosine antibody for 5-Formylcytidine Various BSA conjugated nucleosides were spotted onto PVDF membrane and probed with the antibody at 1:10,000. Column 1: 5-fC. Column. 2: 5-caC. Column. 3: 5-hmC. Column. 4: Cytidine. Column. 5: 5-mC.

产品信息

Tested Applications DB, ICC, IF, IHC, WB

Applications Validated by Active Motif: DB: 1:5,000 dilution
Tested Reactivity Not Species Specific
Host / Isotype Rabbit / Serum
Class Polyclonal
Type Antibody
ModificationMethylated
Immunogen This 5-Formylcytosine antibody was raised against 5-formylcytidine conjugated to KLH and recognizes 5-formylcytosine.
Full Name 5-Formylcytosine (5-fC) antibody (pAb)
Synonyms 5-Formylcytidine, 5-formylcytosine, 5-fC, 5fc, TET, dna methylation, stem cell, stem cells, embryogenesis, sample
Molecular weight
GenBank accession numberN/A
RRIDAB_2687953
Purification Method None
Buffer Rabbit serum containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
Storage Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

背景介绍

DNA methylation is an epigenetic event in which DNA methyltransferases (DNMTs) catalyze the reaction of a methyl group to the fifth carbon of cytosine in a CpG dinucleotide. This modification helps to control gene expression and is also involved in genomic imprinting, while aberrant DNA methylation is often associated with disease. 5-methylcytosine is a modified base that is found in the DNA of plants and vertebrates. A second type of DNA methylation exists, 5-hydroxymethylcytosine (5-hydroxy methylcytosine, 5-hmC). This results from the enzymatic conversion of 5-methylcytosine into 5-hydroxymethylcytosine by the TET family of cytosine oxygenases. Iterative activity of TET on 5-hydroxymethylcytosine results in the conversion of 5-hmC into 5-formylcytosine and 5-carboxylcytosine. An increase in levels of 5-formylcytosine and 5-carboxylcytosine are detected in the mouse male pronucleus following fertilization, which is gradually diluted by DNA replication.