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验证数据展示

  • WB analysis using 83794-4-RR

    WB analysis using 83794-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83794-4-RR (ALOX5 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovarian cancer using 83794-4-RR

    IHC staining of human ovarian cancer using 83794-4-RR

    Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 83794-4-RR (5 Lipoxygenase antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 83794-4-RR

    Affinity and Kinetic Characterization of 83794-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83794-4-RR against Human 5 Lipoxygenase were performed. The affinity constant is 0.392 nM.

5 Lipoxygenase Recombinant antibody

5 Lipoxygenase Recombinant Antibody for WB, IHC, ELISA
Cat No. 83794-4-RR

产品说明书

CloneNo. 240740A10

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

ALOX5, 5-LO, 5LO, 5-lipoxygenase, 5 LOX

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHL-60 cells, THP-1 cells, human placenta tissue
Positive IHC detected inhuman ovarian cancer
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83794-4-RR targets 5 Lipoxygenase in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原 5 Lipoxygenase fusion protein Ag19503 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 arachidonate 5-lipoxygenase
别名 ALOX5, 5-LO, 5LO, 5-lipoxygenase, 5 LOX
计算分子量 674 aa, 78 kDa
观测分子量 70-78 kDa
GenBank蛋白编号BC130332
基因名称 5 Lipoxygenase
Gene ID (NCBI) 240
RRIDAB_3671385
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP09917
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ALOX5(arachidonate 5-lipoxygenase) is also named as LOG5, 5-LO and belongs to the lipoxygenase family. It catalyzes the first step in leukotriene biosynthesis, and plays a role in inflammatory processes. It is a 78 kDa iron-containing enzyme that translocates from the cytosol to associate with FLAP, and converts arachidonate in two steps into the unstable intermediate LTA4. It is essential for cysteinyl-leukotriene (cys-LT) production, critical mediators in asthma(PMID:12911785).

实验方案

Product Specific Protocols
WB protocol for 5 Lipoxygenase antibody 83794-4-RRDownload protocol
IHC protocol for 5 Lipoxygenase antibody 83794-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols