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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 83794-4-RR (same clone as 83794-4-PBS)

    WB analysis using 83794-4-RR (same clone as 83794-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83794-4-RR (ALOX5 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83794-4-PBS in a different storage buffer formulation.

  • IHC staining of human ovarian cancer using 83794-4-RR (same clone as 83794-4-PBS)

    IHC staining of human ovarian cancer using 83794-4-RR (same clone as 83794-4-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 83794-4-RR (5 Lipoxygenase antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83794-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83794-4-RR

    Affinity and Kinetic Characterization of 83794-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83794-4-RR against Human 5 Lipoxygenase were performed. The affinity constant is 0.392 nM.

5 Lipoxygenase Recombinant antibody, PBS Only

5 Lipoxygenase Uni-rAbTM Recombinant Antibody for WB, IHC, ELISA
Cat No. 83794-4-PBS

产品说明书

CloneNo. 240740A10

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

ALOX5, 5-LO, 5LO, 5-lipoxygenase, 5 LOX

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

83794-4-PBS targets 5 Lipoxygenase in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原 5 Lipoxygenase fusion protein Ag19503 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 arachidonate 5-lipoxygenase
别名 ALOX5, 5-LO, 5LO, 5-lipoxygenase, 5 LOX
计算分子量 674 aa, 78 kDa
观测分子量 70-78 kDa
GenBank蛋白编号BC130332
基因名称 5 Lipoxygenase
Gene ID (NCBI) 240
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP09917
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ALOX5(arachidonate 5-lipoxygenase) is also named as LOG5, 5-LO and belongs to the lipoxygenase family. It catalyzes the first step in leukotriene biosynthesis, and plays a role in inflammatory processes. It is a 78 kDa iron-containing enzyme that translocates from the cytosol to associate with FLAP, and converts arachidonate in two steps into the unstable intermediate LTA4. It is essential for cysteinyl-leukotriene (cys-LT) production, critical mediators in asthma(PMID:12911785).