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A1CF Recombinant antibody

A1CF Uni-rAbTM Recombinant Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

241644F10

Cat no : 84322-4-RR

Print datasheet

Synonyms

APOBEC1CF, APOBEC1 complementation factor, ACF65, ACF64, ACF

验证数据展示

  • WB analysis using 84322-4-RR

    WB analysis using 84322-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84322-4-RR (A1CF antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse colon using 84322-4-RR

    IHC staining of mouse colon using 84322-4-RR

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 84322-4-RR (A1CF antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse colon using 84322-4-RR

    IHC staining of mouse colon using 84322-4-RR

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 84322-4-RR (A1CF antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 84322-4-RR

    Affinity and Kinetic Characterization of 84322-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84322-4-RR against Human A1CF were performed. The affinity constant is 0.191 nM.

经过测试的应用

Positive WB detected inmouse liver tissue, rat liver tissue
Positive IHC detected inmouse colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84322-4-RR targets A1CF in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human
Immunogen A1CF fusion protein Ag22929 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name APOBEC1 complementation factor
Synonyms APOBEC1CF, APOBEC1 complementation factor, ACF65, ACF64, ACF
Calculated Molecular Weight 594 aa, 65 kDa
Observed Molecular Weight65 kDa
GenBank Accession NumberBC054873
Gene Symbol A1CF
Gene ID (NCBI) 29974
RRIDAB_3671864
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ9NQ94
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for A1CF antibody 84322-4-RRDownload protocol
IHC protocol for A1CF antibody 84322-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols