验证数据展示
经过测试的应用
| Positive WB detected in | HepG2 cells, U2OS cells, HEK-293 cells, HeLa cells, K-562 cells, HSC-T6 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
67955-1-Ig targets ABCC5 in WB, ELISA applications and shows reactivity with Human, Rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | Human, Rat |
| 免疫原 | ABCC5 fusion protein Ag31674 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | ATP-binding cassette, sub-family C (CFTR/MRP), member 5 |
| 别名 | ABC33, ABCC5, DKFZp686C1782, EST277145, MOAT C, MOATC, MRP5, pABC11, SMRP |
| 计算分子量 | 161 kDa |
| 观测分子量 | 180-200kDa |
| GenBank蛋白编号 | NM_005688 |
| 基因名称 | ABCC5 |
| Gene ID (NCBI) | 10057 |
| RRID | AB_2918706 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | O15440 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ABCC5, also named as MOAT-C, pABC11, SMRP, and MRP5, belongs to the ABC transporter superfamily, ABCC family, and Conjugate transporter (TC 3.A.1.208) subfamily. ABCC5 acts as a multispecific organic anion pump that can transport nucleotide analogs. ABCC5 functions in the cellular export of its substrate, cyclic nucleotides. This export contributes to the degradation of phosphodiesterases and possibly an elimination pathway for cyclic nucleotides. Studies show that ABCC5 provides resistance to thiopurine anticancer drugs, 6-mercatopurine and thioguanine, and the anti-HIV drug 9-(2-phosphonylmethoxyethyl) adenine. ABCC5 may be involved in resistance to thiopurines in acute lymphoblastic leukemia and antiretroviral nucleoside analogs in HIV-infected patients. Since it is glycosylated, the apparent molecular weight of ABCC5 could be variable, ranging from 161 kDa to 200 kDa (PMID: 10893247; PMID: 15897250; PMID: 31338999).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ABCC5 antibody 67955-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
