CoraLite® Plus 488-conjugated ABCC5 Monoclonal antibody
ABCC5 Monoclonal Antibody for FC (Intra)
Host / Isotype
Mouse / IgG1
Reactivity
Human, Rat
Applications
FC (Intra)
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
2H10F11
验证数据展示
经过测试的应用
Positive FC detected in | MCF-7 cells |
For other applications, we recommend the unconjugated version of this antibody, 67955-1-Ig
推荐稀释比
Application | Dilution |
---|---|
Flow Cytometry (FC) | FC : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL488-67955 targets ABCC5 in FC (Intra) applications and shows reactivity with Human, Rat samples.
Tested Applications | FC (Intra) |
Tested Reactivity | Human, Rat |
Immunogen | ABCC5 fusion protein Ag31674 种属同源性预测 |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Full Name | ATP-binding cassette, sub-family C (CFTR/MRP), member 5 |
Synonyms | ABC33, ABCC5, DKFZp686C1782, EST277145, MOAT C, MOATC, MRP5, pABC11, SMRP |
Calculated Molecular Weight | 161 kDa |
Observed Molecular Weight | 180-190kDa |
GenBank Accession Number | NM_005688 |
Gene Symbol | ABCC5 |
Gene ID (NCBI) | 10057 |
Conjugate | CoraLite® Plus 488 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
Form | Liquid |
Purification Method | Protein G purification |
UNIPROT ID | O15440 |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ABCC5, also named as MOAT-C, pABC11, SMRP and MRP5, belongs to the ABC transporter superfamily, ABCC family and Conjugate transporter (TC 3.A.1.208) subfamily. ABCC5 acts as a multispecific organic anion pump which can transport nucleotide analogs. ABCC5 functions in the cellular export of its substrate, cyclic nucleotides. This export contributes to the degradation of phosphodiesterases and possibly an elimination pathway for cyclic nucleotides. Studies show that ABCC5 provides resistance to thiopurine anticancer drugs, 6-mercatopurine and thioguanine, and the anti-HIV drug 9-(2-phosphonylmethoxyethyl) adenine. ABCC5 may be involved in resistance to thiopurines in acute lymphoblastic leukemia and antiretroviral nucleoside analogs in HIV-infected patients. Alternative splicing of this gene has been detected; however, the complete sequence and translation initiation site is unclear.
实验方案
Product Specific Protocols | |
---|---|
FC protocol for CL Plus 488 ABCC5 antibody CL488-67955 | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |