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验证数据展示

  • FC experiment of HeLa using CL594-67960

    FC experiment of HeLa using CL594-67960

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human ABCE1 (CL594-67960, Clone:1B7E8) (red), or 0.4 ug Mouse IgG2b Isotype Control (CL594-66360-3, Clone: K11B8C4B5) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IF Staining of HeLa using CL594-67960

    IF Staining of HeLa using CL594-67960

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite®594 ABCE1 antibody (CL594-67960, Clone: 1B7E8 ) at dilution of 1:200.

CoraLite®594-conjugated ABCE1 Monoclonal antibody

ABCE1 Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL594-67960

产品说明书

CloneNo. 1B7E8

宿主/亚型

Mouse / IgG2b

种属反应性

human, mouse, rat

应用

IF/ICC, FC (Intra)

别名

RLI, OK/SW-cl.40, EC:3.6.5.-, ATP-binding cassette sub-family E member 1, 2'-5'-oligoadenylate-binding protein

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite®594
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy ABCE1 Ready-To-Use IHC Kit. ABCE1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67960-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-67960 targets ABCE1 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human, mouse, rat
免疫原 ABCE1 fusion protein Ag28493 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 ATP-binding cassette, sub-family E (OABP), member 1
别名 RLI, OK/SW-cl.40, EC:3.6.5.-, ATP-binding cassette sub-family E member 1, 2'-5'-oligoadenylate-binding protein
计算分子量 599 aa, 67 kDa
观测分子量68 kDa
GenBank蛋白编号BC016283
基因名称 ABCE1
Gene ID (NCBI) 6059
RRIDAB_2923935
偶联类型 CoraLite®594 Fluorescent Dye
最大激发/发射波长588 nm / 604 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP61221
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ABCE1 is an ATPase that is a member of the ATP-binding cassette (ABC) transporters superfamily and OABP subfamily, it is an essential and highly conserved protein that is required for both eukaryotic translation initiation as well as ribosome biogenesis.

实验方案

Product Specific Protocols
IF protocol for CL594 ABCE1 antibody CL594-67960Download protocol
FC protocol for CL594 ABCE1 antibody CL594-67960Download protocol
Standard Protocols
Click here to view our Standard Protocols