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验证数据展示

  • FC experiment of HeLa using CL594-67960

    FC experiment of HeLa using CL594-67960

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human ABCE1 (CL594-67960, Clone:1B7E8) (red), or 0.4 ug Mouse IgG2b Isotype Control (CL594-66360-3, Clone: K11B8C4B5) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IF Staining of HeLa using CL594-67960

    IF Staining of HeLa using CL594-67960

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite®594 ABCE1 antibody (CL594-67960, Clone: 1B7E8 ) at dilution of 1:200.

CoraLite®594-conjugated ABCE1 Monoclonal antibody

ABCE1 Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL594-67960
CloneNo. 1B7E8

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Synonyms

RLI, OK/SW-cl.40, EC:3.6.5.-, ATP-binding cassette sub-family E member 1, 2'-5'-oligoadenylate-binding protein

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite®594
Size: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy ABCE1 Ready-To-Use IHC Kit. ABCE1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67960-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-67960 targets ABCE1 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human, mouse, rat
Immunogen ABCE1 fusion protein Ag28493 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name ATP-binding cassette, sub-family E (OABP), member 1
Synonyms RLI, OK/SW-cl.40, EC:3.6.5.-, ATP-binding cassette sub-family E member 1, 2'-5'-oligoadenylate-binding protein
Calculated Molecular Weight 599 aa, 67 kDa
Observed Molecular Weight68 kDa
GenBank Accession NumberBC016283
Gene Symbol ABCE1
Gene ID (NCBI) 6059
RRIDAB_2923935
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP61221
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ABCE1 is an ATPase that is a member of the ATP-binding cassette (ABC) transporters superfamily and OABP subfamily, it is an essential and highly conserved protein that is required for both eukaryotic translation initiation as well as ribosome biogenesis.

实验方案

Product Specific Protocols
IF protocol for CL594 ABCE1 antibody CL594-67960Download protocol
FC protocol for CL594 ABCE1 antibody CL594-67960Download protocol
Standard Protocols
Click here to view our Standard Protocols