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验证数据展示

  • WB analysis using 68254-1-Ig (same clone as 68254-1-PBS)

    WB analysis using 68254-1-Ig (same clone as 68254-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68254-1-Ig (ABL1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68254-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 68254-1-Ig (same clone as 68254-1-PBS)

    IF Staining of HepG2 using 68254-1-Ig (same clone as 68254-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using ABL1 antibody (68254-1-Ig, Clone: 1E7A5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68254-1-PBS in a different storage buffer formulation.

  • IF Staining of U2OS using 68254-1-Ig (same clone as 68254-1-PBS)

    IF Staining of U2OS using 68254-1-Ig (same clone as 68254-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using ABL1 antibody (68254-1-Ig, Clone: 1E7A5 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 68254-1-PBS in a different storage buffer formulation.

  • IF Staining of MCF-7 using 68254-1-Ig (same clone as 68254-1-PBS)

    IF Staining of MCF-7 using 68254-1-Ig (same clone as 68254-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using ABL1 antibody (68254-1-Ig, Clone: 1E7A5 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68254-1-PBS in a different storage buffer formulation.

  • FC experiment of K-562 using 68254-1-Ig (same clone as 68254-1-PBS)

    FC experiment of K-562 using 68254-1-Ig (same clone as 68254-1-PBS)

    1X10^6 K-562 cells were intracellularly stained with 0.4 ug Anti-Human ABL1 (68254-1-Ig, Clone:1E7A5) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2b Isotype Control (66360-3-Ig, Clone: K11B8C4B5) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 68254-1-PBS in a different storage buffer formulation.

ABL1 Monoclonal antibody, PBS Only

ABL1 Monoclonal Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 68254-1-PBS

产品说明书

CloneNo. 1E7A5

宿主/亚型

Mouse / IgG2b

种属反应性

human

应用

WB, IF/ICC, FC (Intra), Indirect ELISA

别名

c ABL, JTK7, EC:2.7.10.2, ABL, Abelson tyrosine-protein kinase 1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

68254-1-PBS targets ABL1 in WB, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, FC (Intra), Indirect ELISA Application Description
经测试反应性 human
免疫原 ABL1 fusion protein Ag26152 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 c-abl oncogene 1, receptor tyrosine kinase
别名 c ABL, JTK7, EC:2.7.10.2, ABL, Abelson tyrosine-protein kinase 1
计算分子量 123 kDa
观测分子量 130 kDa
GenBank蛋白编号NM_005157
基因名称 ABL1
Gene ID (NCBI) 25
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
储存缓冲液 PBS Only
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ABL1, also named as ABL, JTK7, c-ABL and p150, belongs to the protein kinase superfamily, Tyr protein kinase family and ABL subfamily. ABL1 regulates cytoskeleton remodeling during cell differentiation, cell division and cell adhesion. ABL1 localizes to dynamic actin structures, and phosphorylates CRK and CRKL, DOK1, and other proteins controlling cytoskeleton dynamics. It regulates DNA repair potentially by activating the proapoptotic pathway when the DNA damage is too severe to be repaired. ABL1 catalyze the reaction: ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate. A chromosomal aberration involving ABL1 is a cause of chronic myeloid leukemia (CML) [MIM:608232]. Translocation t(9;22)(q34;q11) with BCR. The translocation produces a BCR-ABL found also in acute myeloid leukemia (AML) and acute lymphoblastic leukemia (ALL). The antibody is specific to ABL1.