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CoraLite® Plus 488-conjugated ABL1 Monoclonal antibody

ABL1 Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1E7A5

Cat no : CL488-68254

Print datasheet

Synonyms

ABL, ABL1, c ABL, JTK7, p150, Proto oncogene c Abl, Tyrosine protein kinase ABL1, v abl

验证数据展示

  • IF Staining of MCF-7 using CL488-68254

    IF Staining of MCF-7 using CL488-68254

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using CoraLite® Plus 488 ABL1 antibody (CL488-68254, Clone: 1E7A5 ) at dilution of 1:200.

  • IF Staining of HepG2 using CL488-68254

    IF Staining of HepG2 using CL488-68254

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 ABL1 antibody (CL488-68254, Clone: 1E7A5 ) at dilution of 1:200.

经过测试的应用

Positive IF/ICC detected inHepG2 cells, MCF-7 cells
For other applications, we recommend the unconjugated version of this antibody, 68254-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-68254 targets ABL1 in IF/ICC applications and shows reactivity with Human samples.

Tested Applications IF/ICC Application Description
Tested Reactivity Human
Immunogen ABL1 fusion protein Ag26152 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name c-abl oncogene 1, receptor tyrosine kinase
Synonyms ABL, ABL1, c ABL, JTK7, p150, Proto oncogene c Abl, Tyrosine protein kinase ABL1, v abl
Calculated Molecular Weight 123 kDa
Observed Molecular Weight 140 kDa
GenBank Accession NumberNM_005157
Gene Symbol ABL1
Gene ID (NCBI) 25
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ABL1, also named as ABL, JTK7, c-ABL and p150, belongs to the protein kinase superfamily, Tyr protein kinase family and ABL subfamily. ABL1 regulates cytoskeleton remodeling during cell differentiation, cell division and cell adhesion. ABL1 localizes to dynamic actin structures, and phosphorylates CRK and CRKL, DOK1, and other proteins controlling cytoskeleton dynamics. It regulates DNA repair potentially by activating the proapoptotic pathway when the DNA damage is too severe to be repaired. ABL1 catalyze the reaction: ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate. A chromosomal aberration involving ABL1 is a cause of chronic myeloid leukemia (CML) [MIM:608232]. Translocation t(9;22)(q34;q11) with BCR. The translocation produces a BCR-ABL found also in acute myeloid leukemia (AML) and acute lymphoblastic leukemia (ALL). The antibody is specific to ABL1.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 ABL1 antibody CL488-68254Download protocol
Standard Protocols
Click here to view our Standard Protocols