验证数据展示
经过测试的应用
Positive WB detected in | HEK-293 cells, HeLa cells, mouse brain tissue, HepG2 cells |
Positive IP detected in | HepG2 cells |
Positive IHC detected in | mouse skeletal muscle tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HeLa cells |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:1000-1:8000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
21923-1-AP targets ACC1 in WB, IHC, IF/ICC, IP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.
经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
文献引用应用 | WB, IHC, IF, IP, RIP |
经测试反应性 | human, mouse, rat |
文献引用反应性 | human, mouse, rat, pig, chicken, bovine |
免疫原 | ACC1 fusion protein Ag16452 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | acetyl-Coenzyme A carboxylase alpha |
别名 | ACACA, ACAC, Acetyl CoA carboxylase 1, ACC-alpha, ACCA |
计算分子量 | 2383 aa, 275 kDa |
观测分子量 | 250 kDa |
GenBank蛋白编号 | BC137287 |
基因名称 | Acetyl-CoA Carboxylase 1 |
Gene ID (NCBI) | 31 |
RRID | AB_11042445 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | Q13085 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ACACA(Acetyl-CoA carboxylase 1, ACC), also named as ACAC, ACC1 and ACCA, belongs to the biotin containing enzyme family. It catalyzes the synthesis of malonyl-CoA, which is an intermediate substrate playing a pivotal role in the regulation of fatty acid metabolism and energy production. ACACA is involved in the biosynthesis of fatty acids, and malonyl-CoA produced is used as a building block to extend the chain length of fatty acids by fatty acid synthase (FAS)(PMID:19900410). It has 4 isoforms produced by alternative promoter usage with the molecular weight between 260 kDa and 270 kDa.
实验方案
Product Specific Protocols | |
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WB protocol for ACC1 antibody 21923-1-AP | Download protocol |
IHC protocol for ACC1 antibody 21923-1-AP | Download protocol |
IF protocol for ACC1 antibody 21923-1-AP | Download protocol |
IP protocol for ACC1 antibody 21923-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Cell Res Mannose antagonizes GSDME-mediated pyroptosis through AMPK activated by metabolite GlcNAc-6P | ||
Cell Metab Elevation of JAML Promotes Diabetic Kidney Disease by Modulating Podocyte Lipid Metabolism. | ||
Adv Sci (Weinh) Transcriptional Regulation of De Novo Lipogenesis by SIX1 in Liver Cancer Cells | ||
Hepatology Acetyl-CoA carboxylase α promotion of glucose-mediated fatty acid synthesis enhances survival of hepatocellular carcinoma in mice and patients.
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EMBO J TFAM loss induces nuclear actin assembly upon mDia2 malonylation to promote liver cancer metastasis. | ||
EMBO J Fatty acid synthesis is critical for stem cell pluripotency via promoting mitochondrial fission.
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