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ACC1 Polyclonal antibody

ACC1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 21923-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat and More (3)

应用

WB, IHC, IF/ICC, IP, ELISA and More (2)

ACACA, ACAC, Acetyl CoA carboxylase 1, ACC-alpha, ACCA

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, mouse brain tissue, HepG2 cells
Positive IP detected inHepG2 cells
Positive IHC detected inmouse skeletal muscle tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite® Plus 647 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

21923-1-AP targets ACC1 in WB, IHC, IF/ICC, IP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
文献引用应用WB, IHC, IF, IP, RIP
经测试反应性 human, mouse, rat
文献引用反应性human, mouse, rat, pig, chicken, bovine
免疫原 ACC1 fusion protein Ag16452 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 acetyl-Coenzyme A carboxylase alpha
别名 ACACA, ACAC, Acetyl CoA carboxylase 1, ACC-alpha, ACCA
计算分子量 2383 aa, 275 kDa
观测分子量 250 kDa
GenBank蛋白编号BC137287
基因名称 Acetyl-CoA Carboxylase 1
Gene ID (NCBI) 31
RRIDAB_11042445
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ13085
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ACACA(Acetyl-CoA carboxylase 1, ACC), also named as ACAC, ACC1 and ACCA, belongs to the biotin containing enzyme family. It catalyzes the synthesis of malonyl-CoA, which is an intermediate substrate playing a pivotal role in the regulation of fatty acid metabolism and energy production. ACACA is involved in the biosynthesis of fatty acids, and malonyl-CoA produced is used as a building block to extend the chain length of fatty acids by fatty acid synthase (FAS)(PMID:19900410). It has 4 isoforms produced by alternative promoter usage with the molecular weight between 260 kDa and 270 kDa.

实验方案

Product Specific Protocols
WB protocol for ACC1 antibody 21923-1-APDownload protocol
IHC protocol for ACC1 antibody 21923-1-APDownload protocol
IF protocol for ACC1 antibody 21923-1-APDownload protocol
IP protocol for ACC1 antibody 21923-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cell Res

Mannose antagonizes GSDME-mediated pyroptosis through AMPK activated by metabolite GlcNAc-6P

Authors - Yuan-Li Ai
mouseWB

Cell Metab

Elevation of JAML Promotes Diabetic Kidney Disease by Modulating Podocyte Lipid Metabolism.

Authors - Yi Fu
human,mouseWB

Adv Sci (Weinh)

Transcriptional Regulation of De Novo Lipogenesis by SIX1 in Liver Cancer Cells

Authors - Ling Li
humanWB

Hepatology

Acetyl-CoA carboxylase α promotion of glucose-mediated fatty acid synthesis enhances survival of hepatocellular carcinoma in mice and patients.

Authors - Ming-Da Wang
  • KD Validated
humanWB

EMBO J

TFAM loss induces nuclear actin assembly upon mDia2 malonylation to promote liver cancer metastasis.

Authors - Qichao Huang
mouseWB,IF

EMBO J

Fatty acid synthesis is critical for stem cell pluripotency via promoting mitochondrial fission.

Authors - Lihua Wang
  • KD Validated
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