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CoraLite® Plus 488-conjugated ACC1 Polyclonal antibody
ACC1 Polyclonal Antibody for IF/ICC, FC (Intra)
Host / Isotype
Rabbit / IgG
Reactivity
human, rat
Applications
IF/ICC, FC (Intra)
Conjugate
CoraLite® Plus 488 Fluorescent Dye
验证数据展示
经过测试的应用
Positive IF/ICC detected in | HeLa cells |
Positive FC (Intra) detected in | HeLa cells |
For other applications, we recommend the unconjugated version of this antibody, 21923-1-AP
推荐稀释比
Application | Dilution |
---|---|
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL488-21923 targets ACC1 in IF/ICC, FC (Intra) applications and shows reactivity with human, rat samples.
Tested Applications | IF/ICC, FC (Intra) Application Description |
Tested Reactivity | human, rat |
Immunogen | ACC1 fusion protein Ag16452 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | acetyl-Coenzyme A carboxylase alpha |
Synonyms | ACACA, ACAC, Acetyl CoA carboxylase 1, ACC-alpha, ACCA |
Calculated Molecular Weight | 2383 aa, 275 kDa |
Observed Molecular Weight | 260-270 kDa |
GenBank Accession Number | BC137287 |
Gene Symbol | Acetyl-CoA Carboxylase 1 |
Gene ID (NCBI) | 31 |
RRID | AB_2919185 |
Conjugate | CoraLite® Plus 488 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q13085 |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ACACA(Acetyl-CoA carboxylase 1, ACC), also named as ACAC, ACC1 and ACCA, belongs to the biotin containing enzyme family. It catalyzes the synthesis of malonyl-CoA, which is an intermediate substrate playing a pivotal role in the regulation of fatty acid metabolism and energy production. ACACA is involved in the biosynthesis of fatty acids, and malonyl-CoA produced is used as a building block to extend the chain length of fatty acids by fatty acid synthase (FAS)(PMID:19900410). It has 4 isoforms produced by alternative promoter usage with the molecular weight between 260 kDa and 270 kDa.
实验方案
Product Specific Protocols | |
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IF protocol for CL Plus 488 ACC1 antibody CL488-21923 | Download protocol |
FC protocol for CL Plus 488 ACC1 antibody CL488-21923 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |