验证数据展示
经过测试的应用
| Positive IF-P detected in | human liver cancer tissue |
For other applications, we recommend the unconjugated version of this antibody, 67742-1-Ig
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-67742 targets ACADM in IF-P applications and shows reactivity with Human samples.
| 经测试应用 | IF-P Application Description |
| 经测试反应性 | Human |
| 免疫原 | ACADM fusion protein Ag30620 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | acyl-Coenzyme A dehydrogenase, C-4 to C-12 straight chain |
| 别名 | ACAD1, ACADM, FLJ18227, FLJ93013, FLJ99884, MCAD, MCADH |
| 计算分子量 | 421 aa, 47 kDa |
| 观测分子量 | 42-47 kDa |
| GenBank蛋白编号 | BC005377 |
| 基因名称 | ACADM |
| Gene ID (NCBI) | 34 |
| RRID | AB_2919535 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | P11310 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ACADM, also named as MCAD, belongs to the acyl-CoA dehydrogenase family. This enzyme is specific for acyl chain lengths of 4 to 16. It catalyzes the reaction: Acyl-CoA + acceptor = 2,3-dehydroacyl-CoA + reduced acceptor. Defects in ACADM are the cause of medium-chain acyl-CoA dehydrogenase deficiency (MCAD deficiency). This protein can exsit as a dimer(PMID:8962055). This antibody is specific to ACADM.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 ACADM antibody CL488-67742 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |