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CoraLite® Plus 488-conjugated ACADM Monoclonal antibody

ACADM Monoclonal Antibody for IF-P

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

IF-P

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1A11G6

Cat No : CL488-67742

Print datasheet

Synonyms

ACAD1, ACADM, FLJ18227, FLJ93013, FLJ99884, MCAD, MCADH

验证数据展示

  • IF Staining of human liver cancer using CL488-67742

    IF Staining of human liver cancer using CL488-67742

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using CoraLite® Plus 488 ACADM antibody (CL488-67742, Clone: 1A11G6 ) at dilution of 1:200.

经过测试的应用

Positive IF-P detected inhuman liver cancer tissue
For other applications, we recommend the unconjugated version of this antibody, 67742-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67742 targets ACADM in IF-P applications and shows reactivity with Human samples.

Tested Applications IF-P Application Description
Tested Reactivity Human
Immunogen ACADM fusion protein Ag30620 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name acyl-Coenzyme A dehydrogenase, C-4 to C-12 straight chain
Synonyms ACAD1, ACADM, FLJ18227, FLJ93013, FLJ99884, MCAD, MCADH
Calculated Molecular Weight 421 aa, 47 kDa
Observed Molecular Weight42-47 kDa
GenBank Accession NumberBC005377
Gene Symbol ACADM
Gene ID (NCBI) 34
RRIDAB_2919535
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP11310
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ACADM, also named as MCAD, belongs to the acyl-CoA dehydrogenase family. This enzyme is specific for acyl chain lengths of 4 to 16. It catalyzes the reaction: Acyl-CoA + acceptor = 2,3-dehydroacyl-CoA + reduced acceptor. Defects in ACADM are the cause of medium-chain acyl-CoA dehydrogenase deficiency (MCAD deficiency). This protein can exsit as a dimer(PMID:8962055). This antibody is specific to ACADM.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 ACADM antibody CL488-67742Download protocol
Standard Protocols
Click here to view our Standard Protocols