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ACAP1 Recombinant antibody, PBS Only

ACAP1 Recombinant Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

230370D5

Cat No : 82967-4-PBS

Print datasheet

Synonyms

Cnt b1, CENTB1, Centaurin-beta-1, Centaurin beta 1, Arf-GAP with coiled-coil, ANK repeat and PH domain-containing protein 1

验证数据展示

  • WB analysis of Jurkat using 82967-4-RR (same clone as 82967-4-PBS)

    WB analysis of Jurkat using 82967-4-RR (same clone as 82967-4-PBS)

    WB result of ACAP1 antibody (82967-4-RR; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ACAP1 transfected Jurkat cells. This data was developed using the same antibody clone with 82967-4-PBS in a different storage buffer formulation.

  • WB analysis using 82967-4-RR (same clone as 82967-4-PBS)

    WB analysis using 82967-4-RR (same clone as 82967-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82967-4-RR (ACAP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82967-4-PBS in a different storage buffer formulation.

  • IF Staining of Jurkat using 82967-4-RR (same clone as 82967-4-PBS)

    IF Staining of Jurkat using 82967-4-RR (same clone as 82967-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using ACAP1 antibody (82967-4-RR, Clone: 230370D5 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 82967-4-PBS in a different storage buffer formulation.

  • FC experiment of A431 using 82967-4-RR (same clone as 82967-4-PBS)

    FC experiment of A431 using 82967-4-RR (same clone as 82967-4-PBS)

    1x10^6 A431 cells were intracellularly stained with 0.4 ug ACAP1 Recombinant antibody (82967-4-RR, Clone:230370D5) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 82967-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82967-4-RR

    Affinity and Kinetic Characterization of 82967-4-RR

    Biolayer interferometry (BLl) kinetic assays of 82967-4-RR against Human ACAP1 were performed. The affinity constant is below 1 pM.

产品信息

82967-4-PBS targets ACAP1 in WB, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

Tested Applications WB, IF/ICC, FC (Intra), Indirect ELISA Application Description
Tested Reactivity human
Immunogen ACAP1 fusion protein Ag34309 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name ArfGAP with coiled-coil, ankyrin repeat and PH domains 1
Synonyms Cnt b1, CENTB1, Centaurin-beta-1, Centaurin beta 1, Arf-GAP with coiled-coil, ANK repeat and PH domain-containing protein 1
Calculated Molecular Weight 740 aa, 82 kDa
Observed Molecular Weight 82-85 kDa
GenBank Accession NumberBC018543
Gene Symbol ACAP1
Gene ID (NCBI) 9744
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ15027
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ACAP1, also named as Centaurin-beta-1, is a 740 amino acid protein, which is expressed Highly in lung and spleen. ACAP1 as a GTPase-activating protein (GAP) for ADP ribosylation factor 6 (ARF6) is required for clathrin-dependent export of proteins from recycling endosomes to trans-Golgi network and cell surface and required for regulated export of ITGB1 from recycling endosomes to the cell surface and ITGB1-dependent cell migration.