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验证数据展示

  • WB analysis of Jurkat using 82967-4-RR

    WB analysis of Jurkat using 82967-4-RR

    WB result of ACAP1 antibody (82967-4-RR; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ACAP1 transfected Jurkat cells.

  • WB analysis using 82967-4-RR

    WB analysis using 82967-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82967-4-RR (ACAP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of Jurkat using 82967-4-RR

    IF Staining of Jurkat using 82967-4-RR

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using ACAP1 antibody (82967-4-RR, Clone: 230370D5 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • FC experiment of A431 using 82967-4-RR

    FC experiment of A431 using 82967-4-RR

    1x10^6 A431 cells were intracellularly stained with 0.4 ug ACAP1 Recombinant antibody (82967-4-RR, Clone:230370D5) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 82967-4-RR

    Affinity and Kinetic Characterization of 82967-4-RR

    Biolayer interferometry (BLl) kinetic assays of 82967-4-RR against Human ACAP1 were performed. The affinity constant is below 1 pM.

  • Featured Product
  • KD/KO验证

ACAP1 Recombinant antibody

ACAP1 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 82967-4-RR

产品说明书

CloneNo. 230370D5

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, FC (Intra), ELISA

别名

Cnt b1, CENTB1, Centaurin-beta-1, Centaurin beta 1, Arf-GAP with coiled-coil, ANK repeat and PH domain-containing protein 1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inJurkat cells
Positive IF/ICC detected inJurkat cells
Positive FC (Intra) detected inA431 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82967-4-RR targets ACAP1 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原 ACAP1 fusion protein Ag34309 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 ArfGAP with coiled-coil, ankyrin repeat and PH domains 1
别名 Cnt b1, CENTB1, Centaurin-beta-1, Centaurin beta 1, Arf-GAP with coiled-coil, ANK repeat and PH domain-containing protein 1
计算分子量 740 aa, 82 kDa
观测分子量 82-85 kDa
GenBank蛋白编号BC018543
基因名称 ACAP1
Gene ID (NCBI) 9744
RRIDAB_3670714
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ15027
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ACAP1, also named as Centaurin-beta-1, is a 740 amino acid protein, which is expressed Highly in lung and spleen. ACAP1 as a GTPase-activating protein (GAP) for ADP ribosylation factor 6 (ARF6) is required for clathrin-dependent export of proteins from recycling endosomes to trans-Golgi network and cell surface and required for regulated export of ITGB1 from recycling endosomes to the cell surface and ITGB1-dependent cell migration.

实验方案

Product Specific Protocols
WB protocol for ACAP1 antibody 82967-4-RRDownload protocol
IF protocol for ACAP1 antibody 82967-4-RRDownload protocol
FC protocol for ACAP1 antibody 82967-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols