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验证数据展示

  • WB analysis using 80281-2-RR (same clone as 80281-2-PBS)

    WB analysis using 80281-2-RR (same clone as 80281-2-PBS)

    Non-treated NIH/3T33 cells, phosphatase inhibitor treated NIH/3T3 cells and λ phosphatase treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 80281-2-RR (Phospho-ACC1 (Ser79) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80281-2-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 80281-2-RR (same clone as 80281-2-PBS)

    FC experiment of HeLa using 80281-2-RR (same clone as 80281-2-PBS)

    1x10^6 Calyculin A treated HeLa cells were intracellularly stained with 0.25 ug Phospho-ACC1 (Ser79) Recombinant antibody (80281-2-RR, Clone:240235G11) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH. This data was developed using the same antibody clone with 80281-2-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 80281-2-RR (same clone as 80281-2-PBS)

    IF Staining of HeLa using 80281-2-RR (same clone as 80281-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Calyculin A treated HeLa cells using Phospho-ACC1 (Ser79) antibody (80281-2-RR, Clone: 240235G11 ) at dilution of 1:500 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). This data was developed using the same antibody clone with 80281-2-PBS in a different storage buffer formulation.

Phospho-ACC1 (Ser79) Recombinant antibody, PBS Only

Phospho-ACC1 (Ser79) Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 80281-2-PBS

产品说明书

CloneNo. 240235G11

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF/ICC, FC (Intra), ELISA

别名

ACACA, ACC1, ACCA, ACC, ACAC

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

80281-2-PBS targets Phospho-ACC1 (Ser79) in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human, mouse
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 acetyl-Coenzyme A carboxylase alpha
别名 ACACA, ACC1, ACCA, ACC, ACAC
计算分子量 2383 aa, 275 kDa
观测分子量 250-270 kDa
GenBank蛋白编号BC137287
基因名称 Acetyl-CoA Carboxylase 1
Gene ID (NCBI) 31
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ13085
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ACC1 represents a key enzyme, as it is highly regulated by phosphorylation and allosteric regulation, providing a rapid adaptation to new micro-environmental conditions. AMPK phosphorylates acetyl CoA carboxylase (ACC), a rate-controlling step in the conversion of acetyl-CoA to malonyl CoA. This phosphorylation inhibits the activity of ACC, which results in decreased malonyl CoA levels. Additionally, two isoforms of ACC encoded by two different genes in mammalian cells have been described, ACC1 and ACC2. ACC1 is highly enriched in lipogenic tissues (liver and adipose), while ACC2 is mainly expressed in oxidative tissues (heart, skeletal muscle and liver). (PMID: 29056512, PMID: 16054041, PMID: 30816537)