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Species Reactivity
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Conjugate
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Conjugate
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Compatible Species
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验证数据展示

  • WB analysis using 80281-2-RR (same clone as 80281-2-PBS)

    WB analysis using 80281-2-RR (same clone as 80281-2-PBS)

    Non-treated NIH/3T33 cells, phosphatase inhibitor treated NIH/3T3 cells and λ phosphatase treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 80281-2-RR (Phospho-ACC1 (Ser79) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80281-2-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 80281-2-RR (same clone as 80281-2-PBS)

    FC experiment of HeLa using 80281-2-RR (same clone as 80281-2-PBS)

    1x10^6 Calyculin A treated HeLa cells were intracellularly stained with 0.25 ug Phospho-ACC1 (Ser79) Recombinant antibody (80281-2-RR, Clone:240235G11) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH. This data was developed using the same antibody clone with 80281-2-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 80281-2-RR (same clone as 80281-2-PBS)

    IF Staining of HeLa using 80281-2-RR (same clone as 80281-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Calyculin A treated HeLa cells using Phospho-ACC1 (Ser79) antibody (80281-2-RR, Clone: 240235G11 ) at dilution of 1:500 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). This data was developed using the same antibody clone with 80281-2-PBS in a different storage buffer formulation.

Phospho-ACC1 (Ser79) Recombinant antibody, PBS Only

Phospho-ACC1 (Ser79) Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 80281-2-PBS

Print datasheet

CloneNo. 240235G11

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, FC (Intra), ELISA

Synonyms

ACACA, ACC1, ACCA, ACC, ACAC

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size: 

-/ -

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产品信息

80281-2-PBS targets Phospho-ACC1 (Ser79) in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IF/ICC, FC (Intra), ELISA Application Description
Tested Reactivity human, mouse
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name acetyl-Coenzyme A carboxylase alpha
Synonyms ACACA, ACC1, ACCA, ACC, ACAC
Calculated Molecular Weight 2383 aa, 275 kDa
Observed Molecular Weight 250-270 kDa
GenBank Accession NumberBC137287
Gene Symbol Acetyl-CoA Carboxylase 1
Gene ID (NCBI) 31
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ13085
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ACC1 represents a key enzyme, as it is highly regulated by phosphorylation and allosteric regulation, providing a rapid adaptation to new micro-environmental conditions. AMPK phosphorylates acetyl CoA carboxylase (ACC), a rate-controlling step in the conversion of acetyl-CoA to malonyl CoA. This phosphorylation inhibits the activity of ACC, which results in decreased malonyl CoA levels. Additionally, two isoforms of ACC encoded by two different genes in mammalian cells have been described, ACC1 and ACC2. ACC1 is highly enriched in lipogenic tissues (liver and adipose), while ACC2 is mainly expressed in oxidative tissues (heart, skeletal muscle and liver). (PMID: 29056512, PMID: 16054041, PMID: 30816537)