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验证数据展示

  • WB analysis using 80281-2-RR

    WB analysis using 80281-2-RR

    Non-treated NIH/3T33 cells, phosphatase inhibitor treated NIH/3T3 cells and λ phosphatase treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 80281-2-RR (Phospho-ACC1 (Ser79) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • FC experiment of HeLa using 80281-2-RR

    FC experiment of HeLa using 80281-2-RR

    1x10^6 Calyculin A treated HeLa cells were intracellularly stained with 0.25 ug Phospho-ACC1 (Ser79) Recombinant antibody (80281-2-RR, Clone:240235G11) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH.

  • IF Staining of HeLa using 80281-2-RR

    IF Staining of HeLa using 80281-2-RR

    Immunofluorescent analysis of (4% PFA) fixed Calyculin A treated HeLa cells using Phospho-ACC1 (Ser79) antibody (80281-2-RR, Clone: 240235G11 ) at dilution of 1:500 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002).

Phospho-ACC1 (Ser79) Recombinant antibody

Phospho-ACC1 (Ser79) Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 80281-2-RR

产品说明书

CloneNo. 240235G11

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF/ICC, FC (Intra), ELISA

别名

ACC1 (Ser79), ACC1 Ser79, Acetyl Coenzyme A Carboxylase (phospho S79), p ACC1 (Ser79), p-ACC1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inλ phosphatase treated NIH/3T3 cells, NIH/3T3 cells
Positive IF/ICC detected inCalyculin A treated HeLa cells
Positive FC (Intra) detected inCalyculin A treated HeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

80281-2-RR targets Phospho-ACC1 (Ser79) in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human, mouse
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 acetyl-Coenzyme A carboxylase alpha
别名 ACC1 (Ser79), ACC1 Ser79, Acetyl Coenzyme A Carboxylase (phospho S79), p ACC1 (Ser79), p-ACC1
计算分子量 2383 aa, 275 kDa
观测分子量 250-270 kDa
GenBank蛋白编号BC137287
基因名称 Acetyl-CoA Carboxylase 1
Gene ID (NCBI) 31
RRIDAB_3670473
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ13085
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ACC1 represents a key enzyme, as it is highly regulated by phosphorylation and allosteric regulation, providing a rapid adaptation to new micro-environmental conditions. AMPK phosphorylates acetyl CoA carboxylase (ACC), a rate-controlling step in the conversion of acetyl-CoA to malonyl CoA. This phosphorylation inhibits the activity of ACC, which results in decreased malonyl CoA levels. Additionally, two isoforms of ACC encoded by two different genes in mammalian cells have been described, ACC1 and ACC2. ACC1 is highly enriched in lipogenic tissues (liver and adipose), while ACC2 is mainly expressed in oxidative tissues (heart, skeletal muscle and liver). (PMID: 29056512, PMID: 16054041, PMID: 30816537)

实验方案

Product Specific Protocols
WB protocol for Phospho-ACC1 (Ser79) antibody 80281-2-RRDownload protocol
IF protocol for Phospho-ACC1 (Ser79) antibody 80281-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols