验证数据展示
经过测试的应用
Positive WB detected in | λ phosphatase treated NIH/3T3 cells, NIH/3T3 cells |
Positive IF/ICC detected in | Calyculin A treated HeLa cells |
Positive FC (Intra) detected in | Calyculin A treated HeLa cells |
推荐稀释比
Application | Dilution |
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Western Blot (WB) | WB : 1:5000-1:50000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:250-1:1000 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
80281-2-RR targets Phospho-ACC1 (Ser79) in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.
Tested Applications | WB, IF/ICC, FC (Intra), ELISA Application Description |
Tested Reactivity | human, mouse |
Immunogen | Peptide 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Full Name | acetyl-Coenzyme A carboxylase alpha |
Synonyms | ACC1 (Ser79), ACC1 Ser79, Acetyl Coenzyme A Carboxylase (phospho S79), p ACC1 (Ser79), p-ACC1 |
Calculated Molecular Weight | 2383 aa, 275 kDa |
Observed Molecular Weight | 250-270 kDa |
GenBank Accession Number | BC137287 |
Gene Symbol | Acetyl-CoA Carboxylase 1 |
Gene ID (NCBI) | 31 |
RRID | AB_3670473 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purfication |
UNIPROT ID | Q13085 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ACC1 represents a key enzyme, as it is highly regulated by phosphorylation and allosteric regulation, providing a rapid adaptation to new micro-environmental conditions. AMPK phosphorylates acetyl CoA carboxylase (ACC), a rate-controlling step in the conversion of acetyl-CoA to malonyl CoA. This phosphorylation inhibits the activity of ACC, which results in decreased malonyl CoA levels. Additionally, two isoforms of ACC encoded by two different genes in mammalian cells have been described, ACC1 and ACC2. ACC1 is highly enriched in lipogenic tissues (liver and adipose), while ACC2 is mainly expressed in oxidative tissues (heart, skeletal muscle and liver). (PMID: 29056512, PMID: 16054041, PMID: 30816537)
实验方案
Product Specific Protocols | |
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WB protocol for Phospho-ACC1 (Ser79) antibody 80281-2-RR | Download protocol |
IF protocol for Phospho-ACC1 (Ser79) antibody 80281-2-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |