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CoraLite®594-conjugated ACLY Monoclonal antibody

ACLY Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgM

Reactivity

Human, Mouse, Rat

Applications

IF/ICC

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1E10D5

Cat no : CL594-67166

Print datasheet

Synonyms

ACL, ACLY, ATP citrate (pro S ) lyase, ATP citrate lyase, ATP citrate synthase, ATPCL, Citrate cleavage enzyme, CLATP

验证数据展示

  • IF Staining of A549 using CL594-67166

    IF Staining of A549 using CL594-67166

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using CoraLite®594 ACLY antibody (CL594-67166, Clone: 1E10D5 ) at dilution of 1:200.

经过测试的应用

Positive IF/ICC detected inA549 cells
Planning an IHC experiment? We recommend our IHCeasy ACLY Ready-To-Use IHC Kit. ACLY primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67166-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-67166 targets ACLY in IF/ICC applications and shows reactivity with Human, Mouse, Rat samples.

Tested Applications IF/ICC Application Description
Tested Reactivity Human, Mouse, Rat
Immunogen ACLY fusion protein Ag7709 种属同源性预测
Host / Isotype Mouse / IgM
Class Monoclonal
Type Antibody
Full Name ATP citrate lyase
Synonyms ACL, ACLY, ATP citrate (pro S ) lyase, ATP citrate lyase, ATP citrate synthase, ATPCL, Citrate cleavage enzyme, CLATP
Calculated Molecular Weight 121 kDa
Observed Molecular Weight 120 kDa
GenBank Accession NumberBC006195
Gene Symbol ACLY
Gene ID (NCBI) 47
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodCaprylic acid/ammonium sulfate precipitation
UNIPROT IDP53396
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ACLY(ATP-citrate synthase) is also named as ACL. It is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA. ACLY serves as not only a target in oxygenated cells for suppression of lipid synthesis and histone acetylation, but also as a susceptible target in hypoxic cells to restore inhibition of glycolysis. In nonsmall cell lung carcinoma and hepatocellular carcinoma, ACLY is overexpressed compared with normal parenchyma suggesting that ACLY may represent a common target among highly malignant tumors(PMID:19795461). This protein has 2 isoforms produced by alternative splicing.

实验方案

Product Specific Protocols
IF protocol for CL594 ACLY antibody CL594-67166Download protocol
Standard Protocols
Click here to view our Standard Protocols