验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, mouse liver tissue, rat liver tissue |
| Positive IP detected in | HepG2 cells |
| Positive IHC detected in | human stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse liver tissue |
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
12406-1-AP targets Aconitase 1 in WB, IHC, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF-P, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | Aconitase 1 fusion protein Ag3083 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | aconitase 1, soluble |
| 别名 | ACO1, ACO 1, EC:4.2.1.3, Citrate hydro-lyase, Aconitase1 |
| 计算分子量 | 889 aa, 98 kDa |
| 观测分子量 | 98 kDa |
| GenBank蛋白编号 | BC018103 |
| 基因名称 | Aconitase 1 |
| Gene ID (NCBI) | 48 |
| RRID | AB_10642942 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P21399 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Mammalian cells possess two distinct isoforms of aconitase encoded by different genes: Aconitase 1 [ACO1; cytosolic aconitase (c-aconitase)] encoded by ACO1 and Aconitase 2 [ACO2; mitochondrial aconitase (m-aconitase)] encoded by ACO2. In addition to the enzymatic roles in the cytoplasm, ACO1 also is known to bind to mRNAs of specific genes and maintain iron homeostasis.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Aconitase 1 antibody 12406-1-AP | Download protocol |
| IHC protocol for Aconitase 1 antibody 12406-1-AP | Download protocol |
| IF protocol for Aconitase 1 antibody 12406-1-AP | Download protocol |
| IP protocol for Aconitase 1 antibody 12406-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
iScience Asparagine starvation suppresses histone demethylation through iron depletion
| ||
Biochim Biophys Acta Mol Basis Dis Iron deposition-induced ferroptosis in alveolar type II cells promotes the development of pulmonary fibrosis. | ||
Cell Biosci Mutant huntingtin induces iron overload via up-regulating IRP1 in Huntington's disease. | ||
Drug Des Devel Ther Curcumin induces apoptosis and protective autophagy in castration-resistant prostate cancer cells through iron chelation. | ||
Biomolecules Carbamoyl-Phosphate Synthase 1 as a Novel Target of Phomoxanthone A, a Bioactive Fungal Metabolite. | ||
JOJ Ophthalmol Mitophagy, Ferritinophagy and Ferroptosis in Retinal Pigment Epithelial Cells Under High Glucose Conditions: Implications for Diabetic Retinopathy and Age-Related Retinal Diseases. |
