验证数据展示
经过测试的应用
| Positive IF-P detected in | mouse liver tissue |
For other applications, we recommend the unconjugated version of this antibody, 12406-1-AP
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-12406 targets Aconitase 1 in IF-P applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | IF-P Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 | Aconitase 1 fusion protein Ag3083 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | aconitase 1, soluble |
| 别名 | ACO1, Aconitase, aconitase 1, soluble, ACONS, Citrate hydro lyase, Ferritin repressor protein, IRE BP 1, IREB1, IREBP, IREBP1, Iron regulatory protein 1, IRP1 |
| 计算分子量 | 889 aa, 98 kDa |
| 观测分子量 | 98 kDa |
| GenBank蛋白编号 | BC018103 |
| 基因名称 | Aconitase 1 |
| Gene ID (NCBI) | 48 |
| RRID | AB_3083926 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P21399 |
| 储存缓冲液 | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Mammalian cells possess two distinct isoforms of aconitase encoded by different genes: Aconitase 1 [ACO1; cytosolic aconitase (c-aconitase)] encoded by ACO1 and Aconitase 2 [ACO2; mitochondrial aconitase (m-aconitase)] encoded by ACO2. In addition to the enzymatic roles in the cytoplasm, ACO1 also is known to bind to mRNAs of specific genes and maintain iron homeostasis.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 Aconitase 1 antibody CL488-12406 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |