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验证数据展示

  • IF Staining of mouse liver using CL488-12406

    IF Staining of mouse liver using CL488-12406

    Immunofluorescent analysis of (4% PFA) fixed mouse liver tissue using CoraLite® Plus 488 Aconitase 1 antibody (CL488-12406) at dilution of 1:200.

  • IF Staining of mouse liver using CL488-12406

    IF Staining of mouse liver using CL488-12406

    Immunofluorescent analysis of (4% PFA) fixed mouse liver tissue using CoraLite® Plus 488 Aconitase 1 antibody (CL488-12406) at dilution of 1:200.

  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated Aconitase 1 Polyclonal antibody

Aconitase 1 Polyclonal Antibody for IF-P
Cat No. CL488-12406

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF-P

Synonyms

ACO1, Aconitase, aconitase 1, soluble, ACONS, Citrate hydro lyase, Ferritin repressor protein, IRE BP 1, IREB1, IREBP, IREBP1, Iron regulatory protein 1, IRP1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive IF-P detected inmouse liver tissue
For other applications, we recommend the unconjugated version of this antibody, 12406-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-12406 targets Aconitase 1 in IF-P applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF-P Application Description
Tested Reactivity human, mouse, rat
Immunogen Aconitase 1 fusion protein Ag3083 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name aconitase 1, soluble
Synonyms ACO1, Aconitase, aconitase 1, soluble, ACONS, Citrate hydro lyase, Ferritin repressor protein, IRE BP 1, IREB1, IREBP, IREBP1, Iron regulatory protein 1, IRP1
Calculated Molecular Weight 889 aa, 98 kDa
Observed Molecular Weight 98 kDa
GenBank Accession NumberBC018103
Gene Symbol Aconitase 1
Gene ID (NCBI) 48
RRIDAB_3083926
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP21399
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Mammalian cells possess two distinct isoforms of aconitase encoded by different genes: Aconitase 1 [ACO1; cytosolic aconitase (c-aconitase)] encoded by ACO1 and Aconitase 2 [ACO2; mitochondrial aconitase (m-aconitase)] encoded by ACO2. In addition to the enzymatic roles in the cytoplasm, ACO1 also is known to bind to mRNAs of specific genes and maintain iron homeostasis.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 Aconitase 1 antibody CL488-12406Download protocol
Standard Protocols
Click here to view our Standard Protocols