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CoraLite®594-conjugated ACO2 Monoclonal antibody

ACO2 Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1F1G4

Cat no : CL594-67509

Print datasheet

Synonyms

aconitase 2, Citrate hydro-lyase, Aconitase2, Aconitase, ACO 2

验证数据展示

  • FC experiment of HeLa using CL594-67509

    FC experiment of HeLa using CL594-67509

    1X10^6 HeLa cells were intracellularly stained with 0.8 ug CoraLite®594 Anti-Human ACO2 (CL594-67509, Clone:1F1G4) (red), or 0.8 ug CoraLite®594 Mouse IgG1 Isotype Control (MOPC-21) (CL594-65124, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy ACO2 Ready-To-Use IHC Kit. ACO2 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67509-1-Ig

推荐稀释比

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-67509 targets ACO2 in FC (Intra) applications and shows reactivity with human, mouse, rat, pig samples.

Tested Applications FC (Intra) Application Description
Tested Reactivity human, mouse, rat, pig
Immunogen ACO2 fusion protein Ag17784 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name aconitase 2, mitochondrial
Synonyms aconitase 2, Citrate hydro-lyase, Aconitase2, Aconitase, ACO 2
Calculated Molecular Weight 85 kDa
Observed Molecular Weight 85 kDa
GenBank Accession NumberBC014092
Gene Symbol ACO2
Gene ID (NCBI) 50
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ99798
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ACO2(aconitate hydratase, mitochondrial) is also named as citrate hydro-lyase and belongs to the aconitase/IPM isomerase family. It plays a key function in cellular energy production, and loss of its activity has a major impact on cellular and organismal survival. Western blot shows two bands of 83 kDa and 40 kDa. The 40 kDa fragment decreases with age and oxidative stress, whereas other fragmentation products with molecular weights between 40 and 83 kDa increased with age and MnSOD(mitochondrial manganese superoxide dismutase) deficiency(PMID:12459471). Defects in ACO2 are the cause of infantile cerebellar-retinal degeneration (ICRD).

实验方案

Product Specific Protocols
FC protocol for CL594 ACO2 antibody CL594-67509Download protocol
Standard Protocols
Click here to view our Standard Protocols