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  • Featured Product
  • KD/KO Validated

ACOX1 Monoclonal antibody, PBS Only

ACOX1 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

3E12E10

Cat no : 68017-1-PBS

Print datasheet

Synonyms

Palmitoyl-CoA oxidase, EC:1.3.3.6, AOX, ACOX 1, ACOX

验证数据展示

  • WB analysis using 68017-1-Ig (same clone as 68017-1-PBS)

    WB analysis using 68017-1-Ig (same clone as 68017-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68017-1-Ig (ACOX1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68017-1-PBS in a different storage buffer formulation.

  • WB analysis of rat liver using 68017-1-Ig (same clone as 68017-1-PBS)

    WB analysis of rat liver using 68017-1-Ig (same clone as 68017-1-PBS)

    rat liver tissue were subjected to SDS PAGE followed by western blot with 68017-1-Ig (ACOX1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68017-1-PBS in a different storage buffer formulation.

  • WB analysis of HepG2 using 68017-1-Ig (same clone as 68017-1-PBS)

    WB analysis of HepG2 using 68017-1-Ig (same clone as 68017-1-PBS)

    WB result of ACOX1 antibody (68017-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ACOX1 transfected HepG2 cells. This data was developed using the same antibody clone with 68017-1-PBS in a different storage buffer formulation.

  • WB analysis of pig liver using 68017-1-Ig (same clone as 68017-1-PBS)

    WB analysis of pig liver using 68017-1-Ig (same clone as 68017-1-PBS)

    pig liver tissue were subjected to SDS PAGE followed by western blot with 68017-1-Ig (ACOX1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68017-1-PBS in a different storage buffer formulation.

产品信息

68017-1-PBS targets ACOX1 in WB, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human, mouse, rat, pig
Immunogen ACOX1 fusion protein Ag28037 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name acyl-Coenzyme A oxidase 1, palmitoyl
Synonyms Palmitoyl-CoA oxidase, EC:1.3.3.6, AOX, ACOX 1, ACOX
Calculated Molecular Weight 74 kDa
Observed Molecular Weight 50 kDa
GenBank Accession NumberBC010425
Gene Symbol ACOX1
Gene ID (NCBI) 51
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ15067
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ACOX1(Peroxisomal acyl-coenzyme A oxidase 1) is the first and rate-limiting enzyme in the peroxisomal fatty acid beta-oxidation pathway and catalyzes the desaturation of acyl-CoAs to 2-trans-enoyl-CoAs. It belongs to the acyl-CoA oxidase family and also named as ACOX, AOX, SCOX. It has 3 isoforms produced by alternative splicing with the molecular mass of 70-74 kDa and can be cleaved post-translationally into a 50-kDa and a 22-kDa subunit between Val468 and Ala469 (PMID: 10672038). Defects in ACOX1 are the cause of adrenoleukodystrophy pseudoneonatal (Pseudo-NALD).