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验证数据展示

  • IF Staining of HepG2 using CL488-83731

    IF Staining of HepG2 using CL488-83731

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 ACOX1 antibody (CL488-83731, Clone: 240754D3 ) at dilution of 1:200, CL594-Phalloidin (red).

CoraLite® Plus 488-conjugated ACOX1 Recombinant antibody

ACOX1 Recombinant Antibody for IF/ICC
Cat No. CL488-83731

产品说明书

CloneNo. 240754D3

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC

别名

Palmitoyl-CoA oxidase, EC:1.3.3.6, AOX, ACOX 1, ACOX

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 83731-1-PBS

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-83731 targets ACOX1 in IF/ICC applications and shows reactivity with human samples.

经测试应用 IF/ICC Application Description
经测试反应性 human
免疫原 ACOX1 fusion protein Ag1405 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 acyl-Coenzyme A oxidase 1, palmitoyl
别名 Palmitoyl-CoA oxidase, EC:1.3.3.6, AOX, ACOX 1, ACOX
计算分子量 74 kDa
观测分子量70-74 kDa, 22 kDa
GenBank蛋白编号BC010425
基因名称 ACOX1
Gene ID (NCBI) 51
RRIDAB_3673296
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ15067
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ACOX1(Peroxisomal acyl-coenzyme A oxidase 1) is the first and rate-limiting enzyme in the peroxisomal fatty acid beta-oxidation pathway and catalyzes the desaturation of acyl-CoAs to 2-trans-enoyl-CoAs. It belongs to the acyl-CoA oxidase family and also named as ACOX, AOX, SCOX. It has 3 isoforms produced by alternative splicing with the molecular mass of 70-74 kDa and can be cleaved post-translationally into a 50-kDa and a 22-kDa subunit between Val468 and Ala469 (PMID: 10672038). Defects in ACOX1 are the cause of adrenoleukodystrophy pseudoneonatal (Pseudo-NALD).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 ACOX1 antibody CL488-83731Download protocol
Standard Protocols
Click here to view our Standard Protocols