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ACPP Monoclonal antibody

ACPP Monoclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, rat

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

1G2C9

Cat no : 60176-1-Ig

Print datasheet

Synonyms

acid phosphatase, prostate, ACP 3, ACP3, ACPP, PAP, Prostatic acid phosphatase

验证数据展示

  • WB analysis of K-562 using 60176-1-Ig

    WB analysis of K-562 using 60176-1-Ig

    K-562 cells were subjected to SDS PAGE followed by western blot with 60176-1-Ig (ACPP Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IF Staining of human prostate cancer using 60176-1-Ig

    IF Staining of human prostate cancer using 60176-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human prostate cancer tissue using ACPP antibody (60176-1-Ig, Clone: 1G2C9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • IF Staining of human prostate cancer using 60176-1-Ig

    IF Staining of human prostate cancer using 60176-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human prostate cancer tissue using ACPP antibody (60176-1-Ig, Clone: 1G2C9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • IHC staining of human prostate hyperplasia using 60176-1-Ig

    IHC staining of human prostate hyperplasia using 60176-1-Ig

    Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 60176-1-Ig (ACPP antibody) at dilution of 1:1000 (under 10x lens).

    Ready-to-use IHC kits available with PAP/ACPP antibody included
  • IHC staining of human prostate hyperplasia using 60176-1-Ig

    IHC staining of human prostate hyperplasia using 60176-1-Ig

    Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 60176-1-Ig (ACPP antibody) at dilution of 1:1000 (under 40x lens).

    Ready-to-use IHC kits available with PAP/ACPP antibody included

经过测试的应用

Positive WB detected inK-562 cells, human testis tissue
Positive IHC detected inhuman prostate hyperplasia tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman prostate cancer tissue
Planning an IHC experiment? We recommend our IHCeasy PAP/ACPP Ready-To-Use IHC Kit. PAP/ACPP primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

60176-1-Ig targets ACPP in WB, IHC, IF-P, ELISA applications and shows reactivity with human, rat samples.

Tested Applications WB, IHC, IF-P, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, rat
Cited Reactivityhuman
Immunogen ACPP fusion protein Ag8578 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name acid phosphatase, prostate
Synonyms acid phosphatase, prostate, ACP 3, ACP3, ACPP, PAP, Prostatic acid phosphatase
Calculated Molecular Weight 418 aa, 48 kDa
Observed Molecular Weight 45-50 kDa
GenBank Accession NumberBC007460
Gene Symbol ACPP
Gene ID (NCBI) 55
RRIDAB_10733336
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP15309
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ACPP is also named as PAP(prostatic acid phosphatase),5'-nucleotidase,thiamine monophosphatas and Belongs to the histidine acid phosphatase family.This enzyme is a homodimeric glycoprotein with a subunit molecular mass of 50 kDa and is an androgen-stimulated secretory product of the human prostate gland(PMID:8132635).It is also a non-specific phosphatase and has been implicated in the progression of prostate cancer, which inactivates LPA in human seminal plasma(PMID:15280042). This protein has three glycosylation sites(PMID:10639192;12525165).

实验方案

Product Specific Protocols
WB protocol for ACPP antibody 60176-1-IgDownload protocol
IHC protocol for ACPP antibody 60176-1-IgDownload protocol
IF protocol for ACPP antibody 60176-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Front Endocrinol (Lausanne)

Proteomic Analysis of the Alterations in Follicular Fluid Proteins During Oocyte Maturation in Humans.

Authors - Chong Wang
View Article