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CoraLite® Plus 488-conjugated ACPP Monoclonal antibody

ACPP Monoclonal Antibody for IF-P

Host / Isotype

Mouse / IgG2a

Reactivity

human, rat

Applications

IF-P

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1G2C9

Cat No : CL488-60176

Print datasheet

Synonyms

acid phosphatase, prostate, ACP 3, ACP3, ACPP, PAP, Prostatic acid phosphatase

验证数据展示

  • IF Staining of human prostate cancer using CL488-60176

    IF Staining of human prostate cancer using CL488-60176

    Immunofluorescent analysis of (4% PFA) fixed human prostate cancer tissue using CoraLite® Plus 488 ACPP antibody (CL488-60176, Clone: 1G2C9 ) at dilution of 1:200.

经过测试的应用

Positive IF detected inhuman prostate cancer tissue
Planning an IHC experiment? We recommend our IHCeasy PAP/ACPP Ready-To-Use IHC Kit. PAP/ACPP primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 60176-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-60176 targets ACPP in IF applications and shows reactivity with human, rat samples.

Tested Applications IF-P
Tested Reactivity human, rat
Immunogen ACPP fusion protein Ag8578 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name acid phosphatase, prostate
Synonyms acid phosphatase, prostate, ACP 3, ACP3, ACPP, PAP, Prostatic acid phosphatase
Calculated Molecular Weight 418 aa, 48 kDa
Observed Molecular Weight 45-50 kDa
GenBank Accession NumberBC007460
Gene Symbol ACPP
Gene ID (NCBI) 55
RRIDAB_2919233
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP15309
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage.

背景介绍

ACPP is also named as PAP(prostatic acid phosphatase),5'-nucleotidase,thiamine monophosphatas and Belongs to the histidine acid phosphatase family.This enzyme is a homodimeric glycoprotein with a subunit molecular mass of 50 kDa and is an androgen-stimulated secretory product of the human prostate gland(PMID:8132635).It is also a non-specific phosphatase and has been implicated in the progression of prostate cancer, which inactivates LPA in human seminal plasma(PMID:15280042). This protein has three glycosylation sites(PMID:10639192;12525165).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 ACPP antibody CL488-60176Download protocol
Standard Protocols
Click here to view our Standard Protocols