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ACSF2 Recombinant antibody, PBS Only (Detector)

ACSF2 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241208D9

Cat no : 84033-3-PBS

Print datasheet

Synonyms

UNQ493/PRO1009, Medium-chain acyl-CoA ligase ACSF2, mitochondrial, EC:6.2.1.2, ACSF 2, 241208D9

验证数据展示

  • WB analysis using 84033-3-RR (same clone as 84033-3-PBS)

    WB analysis using 84033-3-RR (same clone as 84033-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84033-3-RR (ACSF2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84033-3-PBS in a different storage buffer formulation.

  • FC experiment of A549 using 84033-3-RR (same clone as 84033-3-PBS)

    FC experiment of A549 using 84033-3-RR (same clone as 84033-3-PBS)

    1x10^6 A549 cells were intracellularly stained with 0.25 ug ACSF2 Recombinant antibody (84033-3-RR, Clone:241208D9) and Multi-rAb CoraLite ® Plus 647-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR005)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84033-3-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00952-1

    Cytometric bead array standard curve of MP00952-1

    Cytometric bead array standard curve of MP00952-1, ACSF2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84033-2-PBS. Detection antibody: 84033-3-PBS. Standard: Ag9199. Range: 0.625-80 ng/mL.

  • Cytometric bead array standard curve of MP00952-3

    Cytometric bead array standard curve of MP00952-3

    Cytometric bead array standard curve of MP00952-3, ACSF2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84033-1-PBS. Detection antibody: 84033-3-PBS. Standard: Ag9199. Range: 2.5-80 ng/mL.

产品信息

84033-3-PBS targets ACSF2 as part of a matched antibody pair:

MP00952-1: 84033-2-PBS capture and 84033-3-PBS detection (validated in Cytometric bead array)

MP00952-3: 84033-1-PBS capture and 84033-3-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications WB, FC (Intra), Cytometric bead array, Indirect ELISA Application Description
Tested Reactivity human
Immunogen ACSF2 fusion protein Ag9199 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name acyl-CoA synthetase family member 2
Synonyms UNQ493/PRO1009, Medium-chain acyl-CoA ligase ACSF2, mitochondrial, EC:6.2.1.2, ACSF 2, 241208D9
Calculated Molecular Weight 615 aa, 68 kDa
Observed Molecular Weight51-71 kDa
GenBank Accession NumberBC012053
Gene Symbol ACSF2
Gene ID (NCBI) 80221
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ96CM8
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ACSF2 (encoded by acyl-CoA synthetase family member 2) belongs to the acyl-CoA synthetase (ACS) family, activating fatty acids by forming a thioester bond with CoA.