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验证数据展示

  • FC experiment of HeLa using CL488-19096

    FC experiment of HeLa using CL488-19096

    1X10^6 HeLa cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human ACTN4 (CL488-19096) (red), or 0.8 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated ACTN4 Polyclonal antibody

ACTN4 Polyclonal Antibody for FC (Intra)
Cat No. CL488-19096

Print datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

FC (Intra)

Synonyms

ACTININ 4, actinin, alpha 4, ACTN4, Alpha actinin 4, DKFZp686K23158, F actin cross linking protein, FSGS, FSGS1, Non muscle alpha actinin 4

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive FC (Intra) detected inHeLa cells
Positive FC detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 19096-1-AP

推荐稀释比

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-19096 targets ACTN4 in FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Applications FC (Intra) Application Description
Tested Reactivity human, mouse, rat
Immunogen ACTN4 fusion protein Ag13546 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name actinin, alpha 4
Synonyms ACTININ 4, actinin, alpha 4, ACTN4, Alpha actinin 4, DKFZp686K23158, F actin cross linking protein, FSGS, FSGS1, Non muscle alpha actinin 4
Calculated Molecular Weight 105 kDa
Observed Molecular Weight 100-105 kDa
GenBank Accession NumberBC005033
Gene Symbol ACTN4
Gene ID (NCBI) 81
RRIDAB_3084021
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO43707
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ACTN4 (alpha actinin 4) is a ubiquitous actin-binding protein that cross-links actin filaments into bundles to form filopodia, and has a crucial role in cytoskeletal organization. ACTN4 is colocalized with actin stress fibers and is dispersed in the cytoplasm and in the nucleus. Increased levels of ACTN4 have been associated with cancer metastasis, indicating its implication in cancer cell migration and metastasis. Defects in ACTN4 cause glomerular disease. This antibody recognizes 100-105 kDa of endogenous ACTN4 protein in various lysates. (22370640, 10700177)

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 ACTN4 antibody CL488-19096Download protocol
Standard Protocols
Click here to view our Standard Protocols