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  • Featured Product
  • KD/KO Validated

CoraLite®594-conjugated ACTN4 Monoclonal antibody

ACTN4 Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1E12B8

Cat no : CL594-66628

Print datasheet

Synonyms

ACTININ 4, actinin, alpha 4, ACTN4, Alpha actinin 4, DKFZp686K23158, F actin cross linking protein, FSGS, FSGS1, Non muscle alpha actinin 4

验证数据展示

  • FC experiment of HeLa using CL594-66628

    FC experiment of HeLa using CL594-66628

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human ACTN4 (CL594-66628, Clone:1E12B8) (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

经过测试的应用

Positive FC (Intra) detected inHeLa cells
Positive FC detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 66628-1-Ig

推荐稀释比

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-66628 targets ACTN4 in FC (Intra) applications and shows reactivity with Human, Mouse, Rat samples.

Tested Applications FC (Intra) Application Description
Tested Reactivity Human, Mouse, Rat
Immunogen ACTN4 fusion protein Ag13549 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name actinin, alpha 4
Synonyms ACTININ 4, actinin, alpha 4, ACTN4, Alpha actinin 4, DKFZp686K23158, F actin cross linking protein, FSGS, FSGS1, Non muscle alpha actinin 4
Calculated Molecular Weight 105 kDa
Observed Molecular Weight 105 and 80 kDa
GenBank Accession NumberBC005033
Gene Symbol ACTN4
Gene ID (NCBI) 81
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDO43707
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ACTN4 (alpha actinin 4) is a ubiquitous actin-binding protein that cross-links actin filaments into bundles to form filopodia, and has a crucial role in cytoskeletal organization. ACTN4 is colocalized with actin stress fibers and is dispersed in the cytoplasm and in the nucleus. Increased levels of ACTN4 have been associated with cancer metastasis, indicating its implication in cancer cell migration and metastasis. Defects in ACTN4 cause glomerular disease. This antibody recognizes 105 and 80 kDa of endogenous ACTN4 protein in various lysates. (22370640, 10700177)

实验方案

Product Specific Protocols
FC protocol for CL594 ACTN4 antibody CL594-66628Download protocol
Standard Protocols
Click here to view our Standard Protocols