验证数据展示
经过测试的应用
Positive WB detected in | Calu-3 cells, A549 cells, U2OS cells, HeLa cells, Human peripheral blood platelets, Pig lung tissue, Rabbit lung tissue, Rat lung tissue, Mouse lung tissue |
Positive IHC detected in | mouse brain tissue, mouse spleen tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HUVEC cells |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:5000-1:50000 |
Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
68439-1-Ig targets ARP3/ARP3B in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat, rabbit, pig samples.
经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
经测试反应性 | Human, mouse, rat, rabbit, pig |
免疫原 | ARP3/ARP3B fusion protein Ag5017 种属同源性预测 |
宿主/亚型 | Mouse / IgG1 |
抗体类别 | Monoclonal |
产品类型 | Antibody |
全称 | ARP3 actin-related protein 3 homolog (yeast) |
别名 | Actin like protein 3, Actin related protein 3, ACTR3, ARP3 |
计算分子量 | 418 aa 47 kDa |
观测分子量 | 47 kDa |
GenBank蛋白编号 | BC044590 |
基因名称 | ARP3/ACTR3 |
Gene ID (NCBI) | 10096 |
RRID | AB_3085152 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Protein G purification |
UNIPROT ID | P61158 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ARP3 (also known as ACTR3) is a major constituent of the ARP2/3 complex that is necessary for nucleating actin polymerization at filament branches. ARP3 localizes to the lamellipodia of stationary and locomoting fibroblasts. Overexpression of ARP3 has been reported in several types of cancer cells. This antibody recognizes endogenous ARP3 and may cross-react with ATCR3B.
实验方案
Product Specific Protocols | |
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WB protocol for ARP3/ARP3B antibody 68439-1-Ig | Download protocol |
IHC protocol for ARP3/ARP3B antibody 68439-1-Ig | Download protocol |
IF protocol for ARP3/ARP3B antibody 68439-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |