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CoraLite® Plus 488-conjugated ARP3/ARP3B Monoclonal antibody

ARP3/ARP3B Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig, rabbit

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1D12C7

Cat no : CL488-68439

Print datasheet

Synonyms

验证数据展示

  • IF Staining of HUVEC using CL488-68439

    IF Staining of HUVEC using CL488-68439

    Immunofluorescent analysis of (-20°C Methanol) fixed HUVEC cells using CoraLite® Plus 488 ARP3/ARP3B antibody (CL488-68439, Clone: 1D12C7 ) at dilution of 1:200.

经过测试的应用

Positive IF/ICC detected inHUVEC cells
Planning an IHC experiment? We recommend our IHCeasy ARP3 Ready-To-Use IHC Kit. ARP3 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 68439-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-68439 targets ARP3/ARP3B in IF/ICC applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

Tested Applications IF/ICC Application Description
Tested Reactivity human, mouse, rat, pig, rabbit
Immunogen ARP3/ARP3B fusion protein Ag5017 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name ARP3 actin-related protein 3 homolog (yeast)
Synonyms
Calculated Molecular Weight 418 aa 47 kDa
Observed Molecular Weight 47 kDa
GenBank Accession NumberBC044590
Gene Symbol ARP3/ACTR3
Gene ID (NCBI) 10096
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP61158
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ARP3 (also known as ACTR3) is a major constituent of the ARP2/3 complex that is necessary for nucleating actin polymerization at filament branches. ARP3 localizes to the lamellipodia of stationary and locomoting fibroblasts. Overexpression of ARP3 has been reported in several types of cancer cells. This antibody recognizes endogenous ARP3 and may cross-react with ATCR3B.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 ARP3/ARP3B antibody CL488-68439Download protocol
Standard Protocols
Click here to view our Standard Protocols