验证数据展示
经过测试的应用
| Positive IF/ICC detected in | HUVEC cells |
Planning an IHC experiment? We recommend our IHCeasy ARP3 Ready-To-Use IHC Kit. ARP3 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 68439-1-Ig
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-68439 targets ARP3/ARP3B in IF/ICC applications and shows reactivity with human, mouse, rat, pig, rabbit samples.
| 经测试应用 | IF/ICC Application Description |
| 经测试反应性 | human, mouse, rat, pig, rabbit |
| 免疫原 | ARP3/ARP3B fusion protein Ag5017 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | ARP3 actin-related protein 3 homolog (yeast) |
| 别名 | |
| 计算分子量 | 418 aa 47 kDa |
| 观测分子量 | 47 kDa |
| GenBank蛋白编号 | BC044590 |
| 基因名称 | ARP3/ACTR3 |
| Gene ID (NCBI) | 10096 |
| RRID | AB_3673015 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | P61158 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ARP3 (also known as ACTR3) is a major constituent of the ARP2/3 complex that is necessary for nucleating actin polymerization at filament branches. ARP3 localizes to the lamellipodia of stationary and locomoting fibroblasts. Overexpression of ARP3 has been reported in several types of cancer cells. This antibody recognizes endogenous ARP3 and may cross-react with ATCR3B.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 ARP3/ARP3B antibody CL488-68439 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |