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ACVR1B Recombinant antibody, PBS Only

ACVR1B Uni-rAbTM Recombinant Antibody for WB, IHC, FC (Intra), Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

230260C1

Cat no : 83026-6-PBS

Print datasheet

Synonyms

Activin receptor-like kinase 4, Activin receptor type-1B, Activin receptor type 1B, Activin receptor like kinase 4, activin A receptor, type IB

验证数据展示

  • WB analysis using 83026-6-RR (same clone as 83026-6-PBS)

    WB analysis using 83026-6-RR (same clone as 83026-6-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83026-6-RR (ACVR1B antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83026-6-PBS in a different storage buffer formulation.

  • IHC staining of rat brain using 83026-6-RR (same clone as 83026-6-PBS)

    IHC staining of rat brain using 83026-6-RR (same clone as 83026-6-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 83026-6-RR (ACVR1B antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83026-6-PBS in a different storage buffer formulation.

  • FC experiment of A549 using 83026-6-RR (same clone as 83026-6-PBS)

    FC experiment of A549 using 83026-6-RR (same clone as 83026-6-PBS)

    1x10^6 A549 cells were intracellularly stained with 0.4 ug ACVR1B Recombinant antibody (83026-6-RR, Clone:230260C1) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83026-6-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 83026-6-RR (same clone as 83026-6-PBS)

    IHC staining of mouse brain using 83026-6-RR (same clone as 83026-6-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83026-6-RR (ACVR1B antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83026-6-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83026-6-RR

    Affinity and Kinetic Characterization of 83026-6-RR

    Biolayer interferometry (BLl) kinetic assays of 83026-6-RR against Human ACVR1B were performed. The affinity constant is 35.6 pM.

产品信息

83026-6-PBS targets ACVR1B in WB, IHC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, FC (Intra), Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen ACVR1B fusion protein Ag0129 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name activin A receptor, type IB
Synonyms Activin receptor-like kinase 4, Activin receptor type-1B, Activin receptor type 1B, Activin receptor like kinase 4, activin A receptor, type IB
Calculated Molecular Weight 57 kDa
Observed Molecular Weight52 kDa
GenBank Accession NumberBC000254
Gene Symbol ACVR1B
Gene ID (NCBI) 91
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP36896
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ACVR1B (ALK4, activin A receptor type 1B) is a transforming growth factor-beta (TGF-beta) superfamily member (PMID: 37020544). A potential role for ACVR1B in regulating muscle mass is that it is part of the transforming growth factor b (TGFb) pathway regulating myostatin (PMID: 21063444).