验证数据展示
经过测试的应用
Positive WB detected in | Caco-2 cells, HepG2 cells, HEK-293 cells |
Positive IHC detected in | rat brain tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive FC (Intra) detected in | A549 cells |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:1000-1:4000 |
Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
83026-6-RR targets ACVR1B in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.
经测试应用 | WB, IHC, FC (Intra), ELISA Application Description |
经测试反应性 | human, mouse, rat |
免疫原 | ACVR1B fusion protein Ag0129 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Recombinant |
产品类型 | Antibody |
全称 | activin A receptor, type IB |
别名 | Activin receptor-like kinase 4, Activin receptor type-1B, Activin receptor type 1B, Activin receptor like kinase 4, activin A receptor, type IB |
计算分子量 | 57 kDa |
观测分子量 | 52 kDa |
GenBank蛋白编号 | BC000254 |
基因名称 | ACVR1B |
Gene ID (NCBI) | 91 |
RRID | AB_3670766 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Protein A purification |
UNIPROT ID | P36896 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ACVR1B (ALK4, activin A receptor type 1B) is a transforming growth factor-beta (TGF-beta) superfamily member (PMID: 37020544). A potential role for ACVR1B in regulating muscle mass is that it is part of the transforming growth factor b (TGFb) pathway regulating myostatin (PMID: 21063444).
实验方案
Product Specific Protocols | |
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WB protocol for ACVR1B antibody 83026-6-RR | Download protocol |
IHC protocol for ACVR1B antibody 83026-6-RR | Download protocol |
FC protocol for ACVR1B antibody 83026-6-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |