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验证数据展示

  • WB analysis using 83026-6-RR

    WB analysis using 83026-6-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83026-6-RR (ACVR1B antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of rat brain using 83026-6-RR

    IHC staining of rat brain using 83026-6-RR

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 83026-6-RR (ACVR1B antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • FC experiment of A549 using 83026-6-RR

    FC experiment of A549 using 83026-6-RR

    1x10^6 A549 cells were intracellularly stained with 0.4 ug ACVR1B Recombinant antibody (83026-6-RR, Clone:230260C1) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IHC staining of mouse brain using 83026-6-RR

    IHC staining of mouse brain using 83026-6-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83026-6-RR (ACVR1B antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 83026-6-RR

    Affinity and Kinetic Characterization of 83026-6-RR

    Biolayer interferometry (BLl) kinetic assays of 83026-6-RR against Human ACVR1B were performed. The affinity constant is 35.6 pM.

ACVR1B Recombinant antibody

ACVR1B Recombinant Antibody for WB, IHC, FC (Intra), ELISA
Cat No. 83026-6-RR

Print datasheet

CloneNo. 230260C1

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, FC (Intra), ELISA

Synonyms

Activin receptor-like kinase 4, Activin receptor type-1B, Activin receptor type 1B, Activin receptor like kinase 4, activin A receptor, type IB

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inCaco-2 cells, HepG2 cells, HEK-293 cells
Positive IHC detected inrat brain tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inA549 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83026-6-RR targets ACVR1B in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, FC (Intra), ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen ACVR1B fusion protein Ag0129 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name activin A receptor, type IB
Synonyms Activin receptor-like kinase 4, Activin receptor type-1B, Activin receptor type 1B, Activin receptor like kinase 4, activin A receptor, type IB
Calculated Molecular Weight 57 kDa
Observed Molecular Weight52 kDa
GenBank Accession NumberBC000254
Gene Symbol ACVR1B
Gene ID (NCBI) 91
RRIDAB_3670766
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP36896
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ACVR1B (ALK4, activin A receptor type 1B) is a transforming growth factor-beta (TGF-beta) superfamily member (PMID: 37020544). A potential role for ACVR1B in regulating muscle mass is that it is part of the transforming growth factor b (TGFb) pathway regulating myostatin (PMID: 21063444).

实验方案

Product Specific Protocols
WB protocol for ACVR1B antibody 83026-6-RRDownload protocol
IHC protocol for ACVR1B antibody 83026-6-RRDownload protocol
FC protocol for ACVR1B antibody 83026-6-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols