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验证数据展示

  • IF Staining of Jurkat using 84421-1-RR (same clone as 84421-1-PBS)

    IF Staining of Jurkat using 84421-1-RR (same clone as 84421-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using ACVRL1 antibody (84421-1-RR, Clone: 241686B9 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84421-1-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP01306-2

    Sandwich ELISA standard curve of MP01306-2

    Sandwich ELISA standard curve of MP01306-2, Human ACVRL1 Recombinant Matched Antibody Pair - PBS only. 84421-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg2132. 84421-2-PBS was HRP conjugated as the detection antibody. Range: 0.781-50 ng/mL.

  • Sample test of MP01306-2

    Sample test of MP01306-2

    The mean ACVRL1 concentration was determined to be 34.48 ng/mL in HUVEC cell extract based on a 0.91 mg/mL extract load, 1108.74 ng/mL in HL-60 cell extract based on a 3.00 mg/mL extract load and 176.59 ng/mL in U-937 cell extract based on a 1.40 mg/mL extract load.

  • Cytometric bead array standard curve of MP01306-1

    Cytometric bead array standard curve of MP01306-1

    Cytometric bead array standard curve of MP01306-1, ACVRL1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84421-2-PBS. Detection antibody: 84421-1-PBS. Standard: Eg2132. Range: 0.781-100 ng/mL.

  • Affinity and Kinetic Characterization of 84421-1-PBS

    Affinity and Kinetic Characterization of 84421-1-PBS

    Biolayer interferometry (BLl) kinetic assay of 84421-1-PBS against Human ACVRL1 was performed. The affinity constant is 3.24 nM.

  • Affinity and Kinetic Characterization of 84421-1-RR

    Affinity and Kinetic Characterization of 84421-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84421-1-RR against Human ACVRL1 were performed. The affinity constant is 0.727 nM.

ACVRL1 Recombinant antibody, PBS Only (Capture/Detector)

ACVRL1 Uni-rAbTM Recombinant Antibody for IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 84421-1-PBS

产品说明书

CloneNo. 241686B9

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

别名

ALK1, ALK 1, ACVRLK1, Activin receptor-like kinase 1, Activin receptor like kinase 1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

84421-1-PBS targets ACVRL1 as part of a matched antibody pair:

MP01306-1: 84421-2-PBS capture and 84421-1-PBS detection (validated in Cytometric bead array)

MP01306-2: 84421-1-PBS capture and 84421-2-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test Application Description
经测试反应性 human
免疫原 Recombinant protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 activin A receptor type II-like 1
别名 ALK1, ALK 1, ACVRLK1, Activin receptor-like kinase 1, Activin receptor like kinase 1
计算分子量 56 kDa
GenBank蛋白编号BC042637
基因名称 ACVRL1
Gene ID (NCBI) 94
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP37023
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ACVRL1 (also known as ALK1) is a type I cell-surface receptor for the TGF-beta superfamily of ligands. It shares with other type I receptors a high degree of similarity in serine-threonine kinase subdomains, a glycine- and serine-rich region (called the GS domain) preceding the kinase domain, and a short C-terminal tail. ACVRL1 is highly expressed in endothelial cells and has a critical role in the control of blood vessel development and repair (PMID: 8640225). Mutations in the ACVRL1 gene are associated with hemorrhagic telangiectasia type 2.