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验证数据展示

  • WB analysis using 67870-1-Ig

    WB analysis using 67870-1-Ig

    Jurkat and MOLT-4 cells were subjected to SDS PAGE followed by western blot with 67870-1-Ig (ADA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of Jurkat using 67870-1-Ig

    WB analysis of Jurkat using 67870-1-Ig

    WB result of ADA antibody (67870-1-Ig; 1:40000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ADA transfected Jurkat cells.

  • FC experiment of U2OS using 67870-1-Ig

    FC experiment of U2OS using 67870-1-Ig

    1X10^6 U2OS cells were intracellularly stained with 0.4 ug Anti-Human ADA (67870-1-Ig, Clone:2C4E9) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Featured Product
  • KD/KO验证

ADA Monoclonal antibody

ADA Monoclonal Antibody for WB, ELISA, FC (Intra)
Cat No. 67870-1-Ig

产品说明书

CloneNo. 2C4E9

宿主/亚型

Mouse / IgG2b

种属反应性

Human, rat, pig, rabbit, mouse

应用

WB, ELISA, FC (Intra)

别名

EC:3.5.4.4, ADA1, 2C4E9

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inJurkat cells, LNCaP cells, HEK-293 cells, Sp2/0 cells, rat spleen tissue, rabbit spleen tissue, MOLT-4 cells, pig spleen tissue
Positive FC (Intra) detected inU2OS cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67870-1-Ig targets ADA in WB, ELISA, FC (Intra) applications and shows reactivity with Human, rat, pig, rabbit, mouse samples.

经测试应用 WB, ELISA, FC (Intra) Application Description
经测试反应性 Human, rat, pig, rabbit, mouse
免疫原 ADA fusion protein Ag31326 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 adenosine deaminase
别名 EC:3.5.4.4, ADA1, 2C4E9
计算分子量 363 aa, 41 kDa
观测分子量41 kDa
GenBank蛋白编号BC040226
基因名称 ADA
Gene ID (NCBI) 100
RRIDAB_2918628
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP00813
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ADA antibody 67870-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols