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ADA Monoclonal antibody

ADA Monoclonal Antibody for WB, ELISA, FC (Intra)

Host / Isotype

Mouse / IgG2b

Reactivity

Human, rat, pig, rabbit, mouse

Applications

WB, ELISA, FC (Intra)

Conjugate

Unconjugated

CloneNo.

2C4E9

Cat no : 67870-1-Ig

Print datasheet

Synonyms

EC:3.5.4.4, ADA1, 2C4E9

验证数据展示

  • WB analysis using 67870-1-Ig

    WB analysis using 67870-1-Ig

    Jurkat and MOLT-4 cells were subjected to SDS PAGE followed by western blot with 67870-1-Ig (ADA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of Jurkat using 67870-1-Ig

    WB analysis of Jurkat using 67870-1-Ig

    WB result of ADA antibody (67870-1-Ig; 1:40000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ADA transfected Jurkat cells.

  • FC experiment of U2OS using 67870-1-Ig

    FC experiment of U2OS using 67870-1-Ig

    1X10^6 U2OS cells were intracellularly stained with 0.4 ug Anti-Human ADA (67870-1-Ig, Clone:2C4E9) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive WB detected inJurkat cells, LNCaP cells, HEK-293 cells, Sp2/0 cells, rat spleen tissue, rabbit spleen tissue, MOLT-4 cells, pig spleen tissue
Positive FC (Intra) detected inU2OS cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67870-1-Ig targets ADA in WB, ELISA, FC (Intra) applications and shows reactivity with Human, rat, pig, rabbit, mouse samples.

Tested Applications WB, ELISA, FC (Intra) Application Description
Tested Reactivity Human, rat, pig, rabbit, mouse
Immunogen ADA fusion protein Ag31326 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name adenosine deaminase
Synonyms EC:3.5.4.4, ADA1, 2C4E9
Calculated Molecular Weight 363 aa, 41 kDa
Observed Molecular Weight41 kDa
GenBank Accession NumberBC040226
Gene Symbol ADA
Gene ID (NCBI) 100
RRIDAB_2918628
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP00813
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ADA antibody 67870-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols