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CoraLite® Plus 488-conjugated ADAM12 Polyclonal antibody
ADAM12 Polyclonal Antibody for FC (Intra)
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, Rat
Applications
FC (Intra)
Conjugate
CoraLite® Plus 488 Fluorescent Dye
验证数据展示
For other applications, we recommend the unconjugated version of this antibody, 14139-1-AP
产品信息
CL488-14139 targets ADAM12 in FC (Intra) applications and shows reactivity with human, mouse, Rat samples.
Tested Applications | FC (Intra) Application Description |
Tested Reactivity | human, mouse, Rat |
Immunogen | ADAM12 fusion protein Ag5206 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | ADAM metallopeptidase domain 12 |
Synonyms | ADAM 12, ADAM12, MCMP, MCMPMltna, Meltrin alpha, MLTN, MLTNA |
Calculated Molecular Weight | 100 kDa |
Observed Molecular Weight | 90-100 kDa, 70-80 kDa |
GenBank Accession Number | BC060804 |
Gene Symbol | ADAM12 |
Gene ID (NCBI) | 8038 |
Conjugate | CoraLite® Plus 488 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | O43184 |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ADAM12, also named as MLTN and Meltrin-alpha, is involved in skeletal muscle regeneration, specifically at the onset of cell fusion. It is also involved in macrophage-derived giant cells (MGC) and osteoclast formation from mononuclear precursors. ADAM12 is expressed in human malignant tumors(PMID:17355265). ADAM12's ability to degrade extracellular matrix components likely allows it to detach cancer cells from the basement membrane and assist them on their route to metastasis. But the protein's role not just as a biomarker of breast cancer but as a gateway to cancer cell migration is only now being understood. ADAM12 has 2 isoforms with the calculated molecular mass of 100 and 80 kDa, and this antibody can recognize all isoforms of ADAM12. ADAM12 has 4 forms: 120kDa full-length form, 90kd mature (processed form that lack the prodomain), 50-68kDa degradation product and 27kDa prodomain.
实验方案
Product Specific Protocols | |
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FC protocol for CL Plus 488 ADAM12 antibody CL488-14139 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |