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验证数据展示

  • WB analysis of mouse lung using 22234-1-AP (same clone as 22234-1-PBS)

    WB analysis of mouse lung using 22234-1-AP (same clone as 22234-1-PBS)

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 22234-1-AP (ADAM28 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22234-1-PBS in a different storage buffer formulation.

  • IP experiment of mouse lung using 22234-1-AP (same clone as 22234-1-PBS)

    IP experiment of mouse lung using 22234-1-AP (same clone as 22234-1-PBS)

    IP result of anti-ADAM28 (IP:22234-1-AP, 4ug; Detection:22234-1-AP 1:800) with mouse lung tissue lysate 3600ug. This data was developed using the same antibody clone with 22234-1-PBS in a different storage buffer formulation.

  • WB analysis using 22234-1-AP (same clone as 22234-1-PBS)

    WB analysis using 22234-1-AP (same clone as 22234-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 22234-1-AP (ADAM28 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22234-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

ADAM28 Polyclonal antibody, PBS Only

ADAM28 Polyclonal Antibody for WB, IP, Indirect ELISA
Cat No. 22234-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IP, Indirect ELISA

别名

eMDCII, eMDC II, EC:3.4.24.-, Disintegrin and metalloproteinase domain-containing protein 28, ADAM23

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

22234-1-PBS targets ADAM28 in WB, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IP, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原 ADAM28 fusion protein Ag17586 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ADAM metallopeptidase domain 28
别名 eMDCII, eMDC II, EC:3.4.24.-, Disintegrin and metalloproteinase domain-containing protein 28, ADAM23
计算分子量 775 aa, 87 kDa
观测分子量 35 kDa
GenBank蛋白编号BC136478
基因名称 ADAM28
Gene ID (NCBI) 10863
RRIDAB_2879043
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9UKQ2
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ADAM28 belongs to a family of cell surface and secreted glycoproteins that possess both proteolytic and adhesive properties. ADAM28 is predominantly expressed by carcinoma cells in the breast carcinoma tissues as protein bands of 42 kDa and 55/57 kDa, which correspond to the active forms of ADAM28s and ADAM28m, respectively.In human non-small cell lung carcinomas and breast carcinomas, ADAM28 is overexpressed predominantly by carcinoma cells, and the expression correlates with carcinoma cell proliferation and lymph node metastasis(PMID:19601836).