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ADAM8 Monoclonal antibody

ADAM8 Monoclonal Antibody for WB, FC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, pig

Applications

WB, FC, ELISA

Conjugate

Unconjugated

CloneNo.

3A12H2

Cat no : 68113-1-Ig

Print datasheet

Synonyms

MS2, EC:3.4.24.-, Disintegrin and metalloproteinase domain-containing protein 8, Cell surface antigen MS2, CD156a

验证数据展示

  • WB analysis of human testis using 68113-1-Ig

    WB analysis of human testis using 68113-1-Ig

    human testis tissue were subjected to SDS PAGE followed by western blot with 68113-1-Ig (ADAM8 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis of HaCaT using 68113-1-Ig

    WB analysis of HaCaT using 68113-1-Ig

    HaCaT cells were subjected to SDS PAGE followed by western blot with 68113-1-Ig (ADAM8 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis of A431 using 68113-1-Ig

    WB analysis of A431 using 68113-1-Ig

    A431 cells were subjected to SDS PAGE followed by western blot with 68113-1-Ig (ADAM8 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis using 68113-1-Ig

    WB analysis using 68113-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68113-1-Ig (ADAM8 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • FC experiment of A431 using 68113-1-Ig

    FC experiment of A431 using 68113-1-Ig

    1x10^6 A431 cells were surface stained with 0.8 ug ADAM8 Monoclonal antibody (68113-1-Ig, Clone:3A12H2) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.8 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were not fixed.

经过测试的应用

Positive WB detected inhuman testis tissue, HaCaT cells, A431 cells, pig stomach tissue, pig pancreas tissue
Positive FC detected inA431 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Flow Cytometry (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68113-1-Ig targets ADAM8 in WB, FC, ELISA applications and shows reactivity with human, pig samples.

Tested Applications WB, FC, ELISA Application Description
Tested Reactivity human, pig
Immunogen ADAM8 fusion protein Ag17262 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name ADAM metallopeptidase domain 8
Synonyms MS2, EC:3.4.24.-, Disintegrin and metalloproteinase domain-containing protein 8, Cell surface antigen MS2, CD156a
Calculated Molecular Weight 824 aa, 89 kDa
Observed Molecular Weight90 kDa
GenBank Accession NumberBC115404
Gene Symbol ADAM8
Gene ID (NCBI) 101
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP78325
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ADAM8 is a type I transmembrane (TM) glycoprotein consisting of an N-terminal prodomain, followed by a metalloproteinase-, disintegrin (DIS)-, cysteine-rich-, epidermal growth factor (EGF)-like- and TM domain and a cytoplasmic tail. Expression levels of ADAM8 in normal tissue are typically low and limited to a few distinct cell types in the lymphatic organs as components of the immune system, the central nervous system, bone, and the lung. This antibody can be used to detect endogenous ADAM8 with an apparent molecular weight of 90 kDa (the calculated MW ). ADAM8 also can be detectable as a band with a molecular weight (MW) of 98 kDa, slightly larger than the calculated MW (90 kDa) (PMID:11050116).

实验方案

Product Specific Protocols
WB protocol for ADAM8 antibody 68113-1-IgDownload protocol
FC protocol for ADAM8 antibody 68113-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols