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验证数据展示

  • WB analysis of human testis using 68113-1-Ig

    WB analysis of human testis using 68113-1-Ig

    human testis tissue were subjected to SDS PAGE followed by western blot with 68113-1-Ig (ADAM8 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis of HaCaT using 68113-1-Ig

    WB analysis of HaCaT using 68113-1-Ig

    HaCaT cells were subjected to SDS PAGE followed by western blot with 68113-1-Ig (ADAM8 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis of A431 using 68113-1-Ig

    WB analysis of A431 using 68113-1-Ig

    A431 cells were subjected to SDS PAGE followed by western blot with 68113-1-Ig (ADAM8 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis using 68113-1-Ig

    WB analysis using 68113-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68113-1-Ig (ADAM8 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • FC experiment of A431 using 68113-1-Ig

    FC experiment of A431 using 68113-1-Ig

    1x10^6 A431 cells were surface stained with 0.8 ug ADAM8 Monoclonal antibody (68113-1-Ig, Clone:3A12H2) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.8 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were not fixed.

ADAM8 Monoclonal antibody

ADAM8 Monoclonal Antibody for WB, FC, ELISA
Cat No. 68113-1-Ig

产品说明书

CloneNo. 3A12H2

宿主/亚型

Mouse / IgG1

种属反应性

human, pig

应用

WB, FC, ELISA

别名

MS2, EC:3.4.24.-, Disintegrin and metalloproteinase domain-containing protein 8, Cell surface antigen MS2, CD156a

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inhuman testis tissue, HaCaT cells, A431 cells, pig stomach tissue, pig pancreas tissue
Positive FC detected inA431 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Flow Cytometry (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68113-1-Ig targets ADAM8 in WB, FC, ELISA applications and shows reactivity with human, pig samples.

经测试应用 WB, FC, ELISA Application Description
经测试反应性 human, pig
免疫原 ADAM8 fusion protein Ag17262 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 ADAM metallopeptidase domain 8
别名 MS2, EC:3.4.24.-, Disintegrin and metalloproteinase domain-containing protein 8, Cell surface antigen MS2, CD156a
计算分子量 824 aa, 89 kDa
观测分子量90 kDa
GenBank蛋白编号BC115404
基因名称 ADAM8
Gene ID (NCBI) 101
RRIDAB_2923642
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP78325
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ADAM8 is a type I transmembrane (TM) glycoprotein consisting of an N-terminal prodomain, followed by a metalloproteinase-, disintegrin (DIS)-, cysteine-rich-, epidermal growth factor (EGF)-like- and TM domain and a cytoplasmic tail. Expression levels of ADAM8 in normal tissue are typically low and limited to a few distinct cell types in the lymphatic organs as components of the immune system, the central nervous system, bone, and the lung. This antibody can be used to detect endogenous ADAM8 with an apparent molecular weight of 90 kDa (the calculated MW ). ADAM8 also can be detectable as a band with a molecular weight (MW) of 98 kDa, slightly larger than the calculated MW (90 kDa) (PMID:11050116).

实验方案

Product Specific Protocols
WB protocol for ADAM8 antibody 68113-1-IgDownload protocol
FC protocol for ADAM8 antibody 68113-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols