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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis of human testis using 68113-1-Ig (same clone as 68113-1-PBS)

    WB analysis of human testis using 68113-1-Ig (same clone as 68113-1-PBS)

    human testis tissue were subjected to SDS PAGE followed by western blot with 68113-1-Ig (ADAM8 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68113-1-PBS in a different storage buffer formulation.

  • WB analysis of HaCaT using 68113-1-Ig (same clone as 68113-1-PBS)

    WB analysis of HaCaT using 68113-1-Ig (same clone as 68113-1-PBS)

    HaCaT cells were subjected to SDS PAGE followed by western blot with 68113-1-Ig (ADAM8 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68113-1-PBS in a different storage buffer formulation.

  • WB analysis of A431 using 68113-1-Ig (same clone as 68113-1-PBS)

    WB analysis of A431 using 68113-1-Ig (same clone as 68113-1-PBS)

    A431 cells were subjected to SDS PAGE followed by western blot with 68113-1-Ig (ADAM8 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68113-1-PBS in a different storage buffer formulation.

  • WB analysis using 68113-1-Ig (same clone as 68113-1-PBS)

    WB analysis using 68113-1-Ig (same clone as 68113-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68113-1-Ig (ADAM8 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68113-1-PBS in a different storage buffer formulation.

  • FC experiment of A431 using 68113-1-Ig (same clone as 68113-1-PBS)

    FC experiment of A431 using 68113-1-Ig (same clone as 68113-1-PBS)

    1x10^6 A431 cells were surface stained with 0.8 ug ADAM8 Monoclonal antibody (68113-1-Ig, Clone:3A12H2) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.8 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were not fixed. This data was developed using the same antibody clone with 68113-1-PBS in a different storage buffer formulation.

ADAM8 Monoclonal antibody, PBS Only

ADAM8 Monoclonal Antibody for WB, FC, Indirect ELISA
Cat No. 68113-1-PBS

产品说明书

CloneNo. 3A12H2

宿主/亚型

Mouse / IgG1

种属反应性

human, pig

应用

WB, FC, Indirect ELISA

别名

MS2, EC:3.4.24.-, Disintegrin and metalloproteinase domain-containing protein 8, Cell surface antigen MS2, CD156a

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

68113-1-PBS targets ADAM8 in WB, FC, Indirect ELISA applications and shows reactivity with human, pig samples.

经测试应用 WB, FC, Indirect ELISA Application Description
经测试反应性 human, pig
免疫原 ADAM8 fusion protein Ag17262 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 ADAM metallopeptidase domain 8
别名 MS2, EC:3.4.24.-, Disintegrin and metalloproteinase domain-containing protein 8, Cell surface antigen MS2, CD156a
计算分子量 824 aa, 89 kDa
观测分子量90 kDa
GenBank蛋白编号BC115404
基因名称 ADAM8
Gene ID (NCBI) 101
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP78325
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ADAM8 is a type I transmembrane (TM) glycoprotein consisting of an N-terminal prodomain, followed by a metalloproteinase-, disintegrin (DIS)-, cysteine-rich-, epidermal growth factor (EGF)-like- and TM domain and a cytoplasmic tail. Expression levels of ADAM8 in normal tissue are typically low and limited to a few distinct cell types in the lymphatic organs as components of the immune system, the central nervous system, bone, and the lung. This antibody can be used to detect endogenous ADAM8 with an apparent molecular weight of 90 kDa (the calculated MW ). ADAM8 also can be detectable as a band with a molecular weight (MW) of 98 kDa, slightly larger than the calculated MW (90 kDa) (PMID:11050116).