验证数据展示
经过测试的应用
| Positive WB detected in | mouse placenta tissue, NIH/3T3 cells |
| Positive IF/ICC detected in | NIH/3T3 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
24934-1-AP targets ADAMTS12 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | ADAMTS12 fusion protein Ag20712 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | ADAM metallopeptidase with thrombospondin type 1 motif, 12 |
| 别名 | ADAM TS 12, ADAM TS12, ADAMTS 12, ADAMTS12, PRO4389 |
| 计算分子量 | 178 kDa |
| 观测分子量 | 155 kDa |
| GenBank蛋白编号 | BC058841 |
| 基因名称 | ADAMTS12 |
| Gene ID (NCBI) | 81792 |
| RRID | AB_2879806 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P58397 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ADAMTS12(A disintegrin and metalloproteinase with thrombospondin motifs 12) is also named as AT12, ADAM-TS12, PRO4389 and belongs to the ADAMTS (a disintegrin and metalloproteinase with thrombospondin motifs) protein family. It may play roles in pulmonary cells during fetal development or in tumor processes through its proteolytic activity or as a molecule potentially involved in regulation of cell adhesion. ADAMTS7 and ADAMTS12 appear to be potent negative regulators of endplate cartilage development(PMID:22247065). This protein has 3 isoforms produced by alternative splicing. The full length protein has 14 glycosylation sites.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ADAMTS12 antibody 24934-1-AP | Download protocol |
| IF protocol for ADAMTS12 antibody 24934-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Oncotarget Cleavage of Fibulin-2 by the aggrecanases ADAMTS-4 and ADAMTS-5 contributes to the tumorigenic potential of breast cancer cells. | ||
Hum Mol Genet Epigenetic changes in reterm birth placenta suggest a role for ADAMTS genes in spontaneous preterm birth.
| ||
Cell Signal ADAMTS12 promotes oxaliplatin chemoresistance and angiogenesis in gastric cancer through VEGF upregulation | ||
Sci Rep ADAMTS12 acts as a tumor microenvironment related cancer promoter in gastric cancer.
| ||
Int J Mol Med Overexpression of PRDM13 inhibits glioma cells via Rho and GTP enzyme activation protein. | ||
Hepatobiliary Pancreat Dis Int ADAMTS12 promotes migration and epithelial-mesenchymal transition and predicts poor prognosis for pancreatic cancer.
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