验证数据展示
经过测试的应用
Positive IF/ICC detected in | NIH/3T3 cells |
推荐稀释比
应用 | 推荐稀释比 |
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Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL488-24934 targets ADAMTS12 in IF/ICC applications and shows reactivity with human, mouse samples.
经测试应用 | IF/ICC Application Description |
经测试反应性 | human, mouse |
免疫原 | ADAMTS12 fusion protein Ag20712 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | ADAM metallopeptidase with thrombospondin type 1 motif, 12 |
别名 | ADAM-TS12, ADAM-TS 12, ADAMTS 12, ADAM TS12, ADAM TS 12 |
计算分子量 | 178 kDa |
观测分子量 | 155 kDa |
GenBank蛋白编号 | BC058841 |
基因名称 | ADAMTS12 |
Gene ID (NCBI) | 81792 |
RRID | AB_3672782 |
偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
最大激发/发射波长 | 493 nm / 522 nm |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | P58397 |
储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ADAMTS12(A disintegrin and metalloproteinase with thrombospondin motifs 12) is also named as AT12, ADAM-TS12, PRO4389 and belongs to the ADAMTS (a disintegrin and metalloproteinase with thrombospondin motifs) protein family. It may play roles in pulmonary cells during fetal development or in tumor processes through its proteolytic activity or as a molecule potentially involved in regulation of cell adhesion. ADAMTS7 and ADAMTS12 appear to be potent negative regulators of endplate cartilage development(PMID:22247065). This protein has 3 isoforms produced by alternative splicing. The full length protein has 14 glycosylation sites.
实验方案
Product Specific Protocols | |
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IF protocol for CL Plus 488 ADAMTS12 antibody CL488-24934 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |