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验证数据展示

  • WB analysis using 67764-1-Ig

    WB analysis using 67764-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67764-1-Ig (ADARB1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis of HSC-T6 using 67764-1-Ig

    WB analysis of HSC-T6 using 67764-1-Ig

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67764-1-Ig (ADARB1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis using 67764-1-Ig

    WB analysis using 67764-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67764-1-Ig (ADARB1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

ADARB1 Monoclonal antibody

ADARB1 Monoclonal Antibody for WB, ELISA
Cat No. 67764-1-Ig

产品说明书

CloneNo. 1H8G3

宿主/亚型

Mouse / IgG2b

种属反应性

Human, Mouse, Rat

应用

WB, ELISA

别名

ADAR2, ADAR2a, ADAR2a L1, ADAR2a L2, ADAR2a L3, ADAR2b, ADAR2c, ADAR2d, ADAR2g, ADARB1, DRABA2, DRADA2, dsRNA adenosine deaminase, RED1, RNA editing deaminase 1, RNA editing enzyme 1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected in4T1 cells, HeLa cells, HSC-T6 cells, HepG2 cells, NIH/3T3 cells
Planning an IHC experiment? We recommend our IHCeasy ADARB1 Ready-To-Use IHC Kit. ADARB1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67764-1-Ig targets ADARB1 in WB, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

经测试应用 WB, ELISA Application Description
经测试反应性 Human, Mouse, Rat
免疫原 ADARB1 fusion protein Ag17852 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 adenosine deaminase, RNA-specific, B1 (RED1 homolog rat)
别名 ADAR2, ADAR2a, ADAR2a L1, ADAR2a L2, ADAR2a L3, ADAR2b, ADAR2c, ADAR2d, ADAR2g, ADARB1, DRABA2, DRADA2, dsRNA adenosine deaminase, RED1, RNA editing deaminase 1, RNA editing enzyme 1
计算分子量 741 aa, 81 kDa
观测分子量70 kDa, 80 kDa
GenBank蛋白编号BC065545
基因名称 ADARB1
Gene ID (NCBI) 104
RRIDAB_2918531
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP78563
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ADARB1 (Double-stranded RNA-specific editase 1) is also named as ADAR2, DRADA2, RED1 and belongs to the ADAR family. It catalyses the deamination of adenosine to inosine at the GluR2 Q/R site in the pre-mRNA encoding the critical subunit of AMPA receptors. This protein has some isoforms with the molecular weight from 77 kDa to 81 kDa and 7 kDa, but it can be detected a very strong band at 50 kDa in addition to a predicted band at 75 kDa as the result of western blot, while ADARB1 has several alternative splice variants (PMID:23103828).

实验方案

Product Specific Protocols
WB protocol for ADARB1 antibody 67764-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols